Biotech Articles
Publish Your Research Online
Get Recognition - International Audience

Request for an Author Account   |   Login   |   Submit Article

Micropropagation: Production of Quality Banana Planting

BY: Dilruba Peya | Category: Applications | Submitted: 2013-03-20 22:34:51
       Author Photo
Article Summary: "Banana is a crop which has dual propagation abilities, asexual through suckers and sexual through seeds. Seed propagation is general in wild species that are diploid and go through normal meiosis, seed set and fertilization. The extent of seed set, dormancy and germinability relies on the species..."

Share with Facebook Share with Linkedin Share with Twitter Share with Pinterest Email this article

Banana is a crop which has dual propagation abilities, asexual through suckers and sexual through seeds. Seed propagation is general in wild species that are diploid and go through normal meiosis, seed set and fertilization. The extent of seed set, dormancy and germinability relies on the species. In Ensete, only other genus of the Musaceae, seed propagation is only means of perpetuation as sucker production is not present. Seeds are normally black to brown in color, 3-7 cm in diameter, triangular or round in shape, and mostly compacted in appearance. Fruits of undomesticated species are inedible, being complete of seeds that are covered in thin mucilaginous pulp. All cultivated commercial bananas are sterile and triploid, excepting a small number of parthenocarpic AB and AA diploids. Sucker propagation is only natural means of its perpetuation; artificial processes of propagation are macropropagation and micropropagation.

Sucker propagation

There are two categories of suckers, such as water suckers and sword suckers. Water suckers are less vigorous, small in size, emerge in clumps and broad leaved. Sword suckers have a well developed base, narrow leaf blades and pointed tip. Natural regeneration of the cultivated bananas with suckers is very slow process due to hormone-mediated apical supremacy of the mother plant. A banana plant produces only 6-20 suckers throughout its life span of 10-14 months. For speed up the rate of propagation, suckers with cut rhizomes or growing buds called 'peepers' and 'bits' are used. A number of good bits, every with a centrally located germinating eye can be reduced from an unbunched rhizome following trimming the roots. Selection of proper mother plant for growing new propagules either through in vitro or in vivo methods is significant. About one kg uniformly shaped bits or rhizomes, well-trimmed around the raising sprout are the best beginning material. Although instant planting is ideal, in some parts of the India rhizomes are dried in the sun for 2-3 days after pralinage and paring treatment (trimmed of the all roots, sprinkled with nematicide and dipped in the mud slurry) and stored in shade for seven days before planting.


Macropropagation is an excellent choice for producing low cost planting material. It is a simple process because of the simplicity of multiplication saves cost of the producing planting matter and has the possibility of producing 45-60 shoots per sucker during 4-5 months. Macropropagation is attained by two processes and could be implemented either in the nursery (also called ex-situ condition) or in the field conditions (also called in situ condition). It involves decortication, decapitation and hardening.

a. Decapitation

The Plant of 4-6 month old is headed back, pseudo stem is removed and incisions or cross cuts are made on the raising meristem so that stimulate the generation of lateral buds. This process results in the generation of 10-16 uniform shoots per plant within a short duration of time and is highly appropriate for marginal and small farmers whose needs of planting material are comparatively small. Suckers of selection varieties can be kept in a nursery either in a big concrete pot or in sawdust bed. The primary planting material should preferably be qualified as multiplied at farm level and virus free under a pest proof net house.

b. Decortication

The sword sucker is removed transversely 2 cm on top of the collar region and after that the apical meristem is cut leaving a cavity of 4 cm depth and 2 cm diameter. it is done to overcome apical dominance.

c. Hardening

The lateral sprouts of 7-12 cm length are moved to pro-trays having equal parts vermiculite of and cocopeat and after adequate watering left in a shade net at 80-90 % humidity. Sprouts are generally maintained in pro-trays for a time of 2 to 3 weeks and transferred to polythene bags of thickness of 120 gauge and size 6'x 4' for secondary hardening.

Advantages of Micropropagation for Banana Planting

Micropropagation is the process of quickly multiplying the stock plant material to generate a big number of progeny plants within aseptic conditions utilizing modern plant tissue culture techniques. Application of micropropagation in banana has different advantages, such as, rapid multiplication, production of disease free planting material, season independent production, high returns etc.

The multiplication rate in banana is limited to 5-20 suckers per plant throughout its development period, which makes it hard to obtain adequate amount of planting matter of a clone of selection. Micropropagation facilitates generation of big number of plantlets or unit time, thus assisting in rapid introduction and distribution of new varieties.

The rapid multiplication mechanism makes sure that a small number of mother plants are needed for growing large number of the progeny plants. These mother plants can be preserved with required care at a small cost.

About Author / Additional Info:

Search this site & forums
Share this article with friends:

Share with Facebook Share with Linkedin Share with Twitter Share with Pinterest Email this article

More Social Bookmarks (Digg etc..)

Comments on this article: (0 comments so far)

Comment By Comment

Leave a Comment   |   Article Views: 3819

Additional Articles:

•   Dolly Sheep - The First Cloned Mammal

•   The Role of Carbon in Nanotechnology

•   Role of Pre-breeding in Crop Improvement

•   Biological Control of Insect Pests

Latest Articles in "Applications" category:
•   Flavor Biotechnology: Part -1

•   Flavor Biotechnology: Part -2

•   Genetic Engineering Extended the Shelf-life of Fruits

•   Biomedical Informatics - From Cells to Populations in the IT Way

•   The Concept of Biotechnology: Understanding Various Applications/Uses

•   In Vitro Fertilization Procedure - Applications, Advantages and Disadvantages

•   Fluorescence-Activated Cell Sorting

•   Directed Evolution

•   Fermentation, and its Control

•   Advanced Fermentation Control Strategies

•   Methods of Purification of Enzymes

•   Extremophilic Microbes - Organisms Living in Extreme Conditions

•   Colorful Bacteria

•   Importance of Phytoremediation

•   Conservation of Microbes

•   Sewage Bacteria - Strictly Anaerobic, Aerobic and Facultative bacteria

•   Microbial Growth Substrates

•   Injuries to Microbes

•   Asepsis and its Importance

Important Disclaimer: All articles on this website are for general information only and is not a professional or experts advice. We do not own any responsibility for correctness or authenticity of the information presented in this article, or any loss or injury resulting from it. We do not endorse these articles, we are neither affiliated with the authors of these articles nor responsible for their content. Please see our disclaimer section for complete terms.
Page copy protected against web site content infringement by Copyscape
Copyright © 2010 - Do not copy articles from this website.

Agriculture Bioinformatics Applications Biotech Products Biotech Research
Biology Careers College/Edu DNA Environmental Biotech
Genetics Healthcare Industry News Issues Nanotechnology
Others Stem Cells Press Release Toxicology  

  |   Disclaimer/Privacy/TOS   |   Submission Guidelines   |   Contact Us