Publish Your Research Online
Get Recognition - International Audience
Request for an Author Account | Login | Submit Article
|HOME||FAQ||TOP AUTHORS||FORUMS||PUBLISH ARTICLE|
In Vitro Synthetic Seed Formation in Sugarcane (Saccharum officinarum L.)BY: Muhammad Iqbal | Category: Biotech-Research | Submitted: 2015-11-30 05:50:06
Article Summary: "Synthetic (Artificial) seed production appears a new approach in sugarcane propagation on large scale and helps in maintaining disease free stock. The synthetic seeds with high germination frequency would be useful for commercial scale production of sugarcane plants in future..."
In vitro synthetic seed formation in sugarcane (Saccharum officinarum L.)
Sugarcane (Saccharum officinarum L.) is one of the most important cash crop of the world. Pakistan is the fifth largest sugarcane growing country of the world but our yield per hectare of this crop is lowest than all over the world. There are many reasons for that. The most important of which is the non availability of disease free elite stock for seeding and lack of implementation of advance technologies in sugarcane propagation, development of new seed varieties and maintenance of current promising varieties. In Pakistan sugarcane is propagated by vegetative means and flowering has long been a seriously problem in sugarcane propagation and breeding. Although there are some areas where flowering occur but due to non synchronization of flowering breeding is not possible. Though seeds are produced but mostly they are not fertile. Synthetic seed production in this context thus appears a new approach in sugarcane propagation and maintaining disease free stock.
Three type of explants (leaf, shoot apical meristem and pith) were taken from sugarcane plants and were inoculated to obtain somatic embryos.
Encapsulation of somatic embryos
For encapsulation gel matrix of sodium alginate solution was prepared in the range of 1%, 2%, 2.5%, 3% and 4% in MS medium and calcium chloride solution, as complexing agent was prepared in the range of 100mM, 150mM and 200mM in distilled water. Both the gel matrix and complexing agent (calcium chloride) were autoclaved. Encapsulation was initiated by dropping sodium alginate solution on somatic embryos and then dropping them in the calcium chloride solution so that each drop contained single somatic embryo. The beads were kept for 5 to 25 minutes in the calcium chloride solution for complete polymerization of alginate solution. Finally, these beads were washed three times with sterile distilled water to remove all the traces of calcium chloride. The entire process of encapsulation was carried out in sterile conditions in laminar air flow cabinet.
Synthetic seeds of sugarcane
Storage of encapsulated somatic embryos
Encapsulated somatic embryos or synthetic seeds were stored at 4oC, 8oC, 12oC, 20oC, 24oC, 28oC in sterile distilled water in refrigerator for the duration of 0, 15, 30, 45 and 60 days.
Effect of different temperature conditions on storage of synthetic seeds
Synthetic seeds were stored at different places having different temperature to find the most optimal temperature for storage of synthetic seeds. The storage quality of synthetic seeds was most suitable and appropriate at 4°C temperature and synthetic seeds remained viable at this temperature (Table 1). The storage quality of synthetic seed was good at 8°C temperature. By increasing temperature (12°C - 28°C) the storage quality of synthetic seed was reduced (Table 1).
Pretreatment and germination of synthetic seeds
Before germination, stored synthetic seeds were treated with 200mM KNO3 with gentle shaking for 5 minutes. After rinsing with sterile distilled water they were treated with 1.0 mgl-1 of GA3 for 2 minutes. Its performance was compared with normal synthetic seeds without pretreatment. For germination, synthetic seeds were transferred to the test tubes having MS medium with and without growth regulators. The pH of the medium was adjusted to 5.7-5.8. Cultures were maintained at 25°C with photoperiod of 16/8 h light/dark. The synthetic seeds treated with KNO3 germinated earlier than seeds without pretreatment with KNO3. It took 30 days for sprouting when synthetic seeds were not subjected to pretreatment and germinate within two week when treated with 200mM KNO3.
About Author / Additional Info:
Comments on this article: (0 comments so far)
• Bioremediation - Removal of Toxic Contaminants From the Environment
• Genetic Sexing System: Elimination of a Particular Sex in the Population
• Need for Sugarcane Market Outlook Exercises in India
• Phytoplankton: Ocean Dwellers Who Can Save Our Environment.
Latest Articles in "Biotech-Research" category:
• Human Longevity: A Revolution in Biotechnology and Nanotechnology.
• Nanoparticles as Delivery Device For Gene Therapy
• Biotechnology as a Tool in Medicine: Focus on Artemisinin
• Tissue Cells and Skin Cells Reprogrammed Into Embryonic Stem Cells:-
• Polymerase Chain Reaction (or PCR) - Technique For Amplifying DNA
• Treatment of Heart Disease With Stem Cells
• Biological Activities and Bioassays
• DNA Sequencing: Maxam Gilbert Method
• PCR Aspects and its Future | PCR versus Cloning
• Plasmid as Vectors For Plant Transformation
• Gene Isolation and Characterisation
• Apoptosis and Cancer: A Review
• Extraction of Nucleic Acids (DNA and RNA) From Plant Tissues
• Stem Cells From Bone Marrow and Vein Leftovers Can Heal Damaged Hearts
• Gene Transfer Techniques: Biolistics, Bacterial and Viral Transformation
• Breast Cancer: Cactus For Womens Life
• Mtt Assay: Assess The Viability Of Cell In Culture
• Medicinal Plants: Source Of Medicine
• Biotechnology Impact on Alzheimer's Disease
Important Disclaimer: All articles on this website are for general information only and is not a professional or experts advice. We do not own any responsibility for correctness or authenticity of the information presented in this article, or any loss or injury resulting from it. We do not endorse these articles, we are neither affiliated with the authors of these articles nor responsible for their content. Please see our disclaimer section for complete terms.
Copyright © 2010 biotecharticles.com - Do not copy articles from this website.
ARTICLE CATEGORIES :
| Disclaimer/Privacy/TOS | Submission Guidelines | Contact Us