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Most Recent Biotech Research Articles.
  • In Vitro Synthetic Seed Formation in Sugarcane (Saccharum officinarum L.)    By: Muhammad Iqbal

    Synthetic (Artificial) seed production appears a new approach in sugarcane propagation on large scale and helps in maintaining disease free stock. The synthetic seeds with high germination frequency would be useful for commercial scale production of sugarcane plants in future. >> Category: Biotech Research
  • Scientists Developed a Prototype of Rotaxane Self-replicating System    By: Ada Brown

    Scientists developed a self replicating system for rotaxane, which can be considered as a milestone for the further development of mature self replicating system. >> Category: Biotech Research
  • Plant Pathogen Interactions using Proteomic Approaches    By: Dr. A. Kandan

    Extensive studies of plant pathogen interactions using different proteomic approaches showed differential expression of several protein involved in host metabolic engineering, defense mechanisms etc. In this article, authors briefly discussed expression of several proteins about phytopathogenic fungus, bacteria and biocontrol agents interactions with hosts. >> Category: Biotech Research
  • Functions of miRNAs During Host-Pathogen Interactions - An Overview    By: Dr. A. Kandan

    MicroRNAs (miRNAs) play a major role in regulating the transcription factors of host during the pathogen infection at the early and late phase in host. Here we discussed about the functions of miRNAs during fungal pathogen infection on several hosts and their regulation of gene(s) during pathogenesis. >> Category: Biotech Research
  • Biochip Technology For Multianalyte Detection    By: Nimisha Tehri

    The present article is an attempt to explore the potential of biochip technology employing specific bioreceptors to target multianalyte such as microbial pathogens, pesticides, mycotoxins etc. in samples collected from food, environment and human sources >> Category: Biotech Research
  • DNA Barcoding: For Taxonomy for Species Identification    By: Dr. Jameel Akhtar

    Conventional species identification methods are laborious and time consuming and properly not solve the issue of species identification. Under such situations, DNA-based barcode shows the potential to be a powerful supplement for the phenotypic identification of an organism to species or sub-species level. >> Category: Biotech Research
  • HRMA Method for Sensitive Detection of Plant Pathogens    By: Dr. A. Kandan

    High-Resolution Melting Analysis (HRMA) is relatively a novel technique which is very specific and sensitive in pathogen detection and rapidly identifies in planta infection even when the phytopathogens are present in very small amounts are discussed briefly. >> Category: Biotech Research
  • Biotechnology for Edible Luffa Gourds Improvement    By: Dr. Amish Kumar Sureja

    This article presents an overview of biotechnological applications in edible Luffa gourds improvement. >> Category: Biotech Research
  • Biotechnology for Carrot Improvement    By: Dr. Amish Kumar Sureja

    This article presents an overview of biotechnological applications in carrot improvement. Carrots are valuable for their taste, good digestibility, and high contents of provitamin A, other carotenoids, and fibers. >> Category: Biotech Research
  • Advances in Development of Marker-Free Transgenic Plants    By: Vijaykumar Ishwarbhai Prajapati

    Selectable marker genes (SMGs) and selection agents are useful tools in the production of transgenic plants. Most SMGs express protein products that confer antibiotic- or herbicide resistance traits, and typically reside in the end product of genetically modified (GM) plants. The presence of marker genes in their genomes becomes useless and their effects become unpredictable or even potentially dangerous. In recent years, several strategies have been developed to remove SMGs from GM products while retaining the transgenes of interest. This article describes the existing strategies for SMG removal, including the implementation of recently developed techniques like Mega-nuclease, TALENs, ZFNs and CRISPR. >> Category: Biotech Research
  • DNA Metabarcoding: A Rapid Method of Biodiversity Assessment    By: Dr. Suresh Kaushik

    DNA metabarcoding refers to the automated identification of multiple species from a single bulk sample containing entire organisms. This offers unprecedented scientific and operation opportunities in order to understand biodiversity distribution and dynamics in a better way. >> Category: Biotech Research
  • Allele Mining in Crop Improvement: Prospects and Potentials    By: Kundansingh R. Jadhao

    Allele mining is a promising approach to dissect naturally occurring allelic variation at candidate genes controlling key agronomic traits which has potential applications in crop improvement programs. >> Category: Biotech Research
  • Microbial Metal Bioaccumulation [PDF]    By: Fayaz Ahmad Malla

    Heavy-metal pollution represents an important environmental problem due to the toxic effects of metals, and their accumulation throughout the food chain leads to serious ecological and health problems. Metal remediation through common physico-chemical techniques is expensive and unsuitable in case of voluminous effluents containing complexing organic matter and low metal contamination. Biotechnological approaches that are designed to cover such niches have, therefore, received great deal of attention in the recent years. Biosorption studies involving low-cost and often dead/pre-treated biomass have dominated the literature and, subsequently, extensive reviews focusing on equilibrium and kinetics of metal Biosorption have also come up. >> Category: Biotech Research
  • Faster Breeding Approaches for Enhancing Wheat Production    By: Dr. Vikas Gupta

    This article discusses how different approaches like marker assisted breeding, shuttle breeding and doubled haploid can be used to breed wheat faster to meet the growing challenges of food demand. >> Category: Biotech Research
  • Development of C4 Rice: Current Progress and Future Challenges    By: Gajendra Rathod

    Present article describes about the development and strategies for production of C4 rice. Overproduction of C4 photosynthetic enzymes in transgenic rice plants: >> Category: Biotech Research
  • New Method of Targeting Gene Using CRISPR/cas9 and TALENs    By: Gajendra Rathod

    Novel method of gene targeting using naturally occurring DNA double strand break repair mechanism known as microhomology mediated end joining (MMEJ). >> Category: Biotech Research
  • Functional Molecular Markers: Basic Concept    By: Rakesh Kumar Prajapat

    Different approaches have recently adopted for the functional characterization of allelic variation in plants and to identify sequence motifs affecting phenotypic variation. The term Functional Markers indicate DNA markers derived from functionally characterized sequence motifs. Functional markers are superior to random DNA markers such as RFLPs, SSRs and AFLPs owing to complete linkage with trait locus alleles. >> Category: Biotech Research
  • Phytoremediation: Transgenic Approach    By: Abhilasha Sharma

    This article is mainly focused on the transgenic solutions to obtaining appropriate plants for phytoremediation. The general strategies underlying this approach is to introduce genes conferring ability to tolerance and detoxification of organic and inorganic contaminants/pollutants into larger plants capable of rapid development >> Category: Biotech Research
  • Brief Guide on Rice Proteome    By: Dr. Ashish Vala

    Easy to identify those protein of rice that regulated in response to different stress condition with knowing where and when individual protein may synthesized, with respect to specific stages, and also provide clues to their function using rice proteome database. New technology, promises a great deal for developing the high yield and sustainable agriculture tomorrow. >> Category: Biotech Research
  • EST Based Molecular Markers    By: Chet Ram

    EST databases are the valuable and easily accessible sources for evaluating genomic resources. In the present era of genomics, different types of EST based markers are available and some are in developing phase. >> Category: Biotech Research
  • Development of Doubled Haploids in Wheat    By: Dr. Vikas Gupta

    The doubled haploid technique using Wheat x Maize system is becoming popular as completely homozygous lines can be produced in a single generation as compared to conventional plant breeding. >> Category: Biotech Research
  • A Brief Guide to Marker Free Transgenic Plants    By: Dr. Ashish Vala

    Selectable marker gens have been extraordinarily useful in enabling plant transformation because of the low efficiency of transgene integration. The most used SMGs encode proteins resistant to antibiotics or herbicides and use negative selection , i.e., by killing non transgenic tissue. Transformation using marker not based on antibiotic or herbicide resistance gene, as well as different system of marker gene deletion strategies implemented. These strategies in crops and their further improvement will help to expedite widespread public acceptance of agricultural biotechnology. >> Category: Biotech Research
  • Genome Editing: Conceptual Introduction    By: Bhupendra Singh Panwar

    Genome editing is one of the powerful technology which enable us to modify and correct hereditary information. Tools available for genome editing are Zinc finger nucleas, Transcription activator-like effector nucleas, CRISPR/Cas system and meganucleases. Genome editing increases the efficiency of the gene editing and eventually expands their uses in both basic as well as applied research >> Category: Biotech Research
  • Pathogenicity of Different Aeromonas Spp    By: Sreeremya Sasi

    Aeromonas spp are ubiquitous Gram negative bacilli, now a day classified within the Aeromonadaceae family. The species of this genus have long been known to cause different type of infections in fish, reptiles and amphibians, and some species mainly, A. hydrophila, A sobria and A. caviae have been described as emergent food borne pathogens implicated in human gastroenteritis ranging from mild diarrhea to chlora-like illness. >> Category: Biotech Research
  • Role of KCl and MgCl2 in PCR    By: Subin Raj

    This article summarizes the possible roles of the salts, Potassium Chloride and Magnesium Chloride in the PCR. >> Category: Biotech Research
  • Genes Responsible For Pepper Heat    By: Dr. Arpita Srivastava

    The goal of this article is to present an overview of candidate genes identified to date responsible for pungency in pepper. >> Category: Biotech Research
  • Developing Weed Resistance: Hurdle the Notability of 'Roundup Ready'    By: Dr. Deepak Rameshwar Sapkal

    Glyphosate (Roundup Ready) is a familiar and trusted herbicide to most of the farmers because it was used continuously for nearly two decades to control weeds in non crop areas before introduction of glyphosate resistance. The herbicide glyphosate has so many characteristics that make it an ideal choice for use in resistant crop. >> Category: Biotech Research
  • Role of WRKY Family Proteins in Innate Immunity in Pepper    By: Dr. Manisha Mangal

    Plants activate multiple defense mechanisms against pathogen attack in order to protect themselves from infection. This kind of response is often triggered by the recognition of specific pathogens and the activation of highly regulated signal transduction pathways particularly in the cell nucleus, where signals lead to the transcriptional activation of a large array of defense genes. WRKY transcription factors play essential roles in mediating defense responses to different pathogens in pepper. >> Category: Biotech Research
  • Development of Transgenics for Virus Resistance    By: Dr. Manisha Mangal

    Advancements in transgenic technology has offered new means of incorporating traits for virus resistance into existing desirable crops. There are two approaches for developing transgenics for virus resistance depending on the source of the genes used. The genes can be either from the pathogenic virus itself or from any other source. >> Category: Biotech Research
  • Development of Transgenics for Improved Shelf Life    By: Dr. Manisha Mangal

    The postharvest losses of fruits and vegetables in the developing countries account for almost 50% of the produce. India, the world's second largest producer of fruits and vegetables loses 35-40% of produce because of excessive softening. The softening that accompanies ripening of fruits exacerbates damage during shipping and handling processes. It plays a major role in determining the cost factor, because it has a direct impact on palatability, consumer acceptability, shelf life, and postharvest disease/pathogen resistance. >> Category: Biotech Research
  • Gene Identification Using Candidate Gene Approach in Pepper    By: Dr. Arpita Srivastava

    Candidate gene approach has been applied with much success in pepper and the goal of this article is to present an overview of candidate gene analyses done in pepper for gene identification. >> Category: Biotech Research
  • MiRNA-Induced Gene Silencing (MIGS): For Regulation of Gene Expression    By: Dr. Basavaprabhu L. Patil

    In plants, some miRNAs can trigger production of secondary siRNAs from their targets. miRNA-induced gene silencing (MIGS) is based on this unique feature to efficiently downregulate gene expression. The simple flanking of a sequence of interest with the target site for miR173 is enough to initiate the generation of secondary siRNAs and, subsequently, silencing of the target gene/s. >> Category: Biotech Research
  • Bacillus Thuringiensis - Crystal Proteins For Insect Management    By: Dr. Rohini Sreevathsa

    Plants have to combat various biotic and abiotic stresses during their life. Insects pests are of the serious concerns that reduce yield of important crop species drastically. Plant Biotechnology offers seed-based solutions to mitigate insect pests. One of the ways is through deploying Bacillus thuringiensis insecticidal crystal protein genes through transgenic technology. >> Category: Biotech Research
  • In Vitro Micropropagation and Phytochemical Study of Tinospora Cordifolia    By: Nandan Nair

    Early micropropagation study of Tinospora cordifolia. Micropropagation is widely used technique for the regeneration of whole plant through tissue culture. This has become one of the most beneficial ways to produce species that are difficult to propagate by conventional methods. >> Category: Biotech Research
  • Cancer Tactics and Their Counterstrategies    By: Nazish Mustafa

    The potential cancer treatment is not developed yet neither an appropriate and efficient diagnostic tool for cancer early diagnosis has developed .The breakthrough research in cancer has revealed some of the important facts which answers all those questions about failures of potential cancer treatment and the reasons behind incomplete eradication of cancer from the body. >> Category: Biotech Research
  • Targeted Genome Editing in Plants    By: Dr. Monika Dalal

    The ability to incorporate useful genes and to modify genes in the genomes has been the aim of researchers worldwide. The nuclease based techniques of targeted genome editing have emerged as an efficient tool for precise manipulation of gene sequences in their natural chromosomal context and addition of transgenes to specific genomic loci. >> Category: Biotech Research
  • Genomic Approaches for Engineering of Metabolic Pathways of Medicinal Plants    By: Dr.Vinay Kumar

    This article focused on the functional genomic approaches for engineering of secondary metabolic pathways of medicinal and aromatic plants. Genetic engineering of a secondary metabolic pathway aims to either increase or decrease the quantity of a desired compound or group of compounds for production of novel compounds of medicinal significance. >> Category: Biotech Research
  • Transgenics: Concerns and Prospects    By: Dr. Shiv Kumar

    The article talks about concerns and prospects of transgenics in general. The focus of article is how to restore the confidence and trust of consumer by educating him and what potential benefits can be tapped using this powerful technology in evolving regulatory mechanism of developing countries. >> Category: Biotech Research
  • Molecular Markers and their Utilization in Plant Breeding    By: Dr. Vikas Gupta

    Molecular markers usage now a days in Plant breeding is a routine activity. A brief introduction about molecular markers and their utilization in plant breeding is discussed. >> Category: Biotech Research
  • Glycomics - An Overview of the Carbohydrate Study    By: Divya Narayan

    Glycomics not only involves the study of carbohydrates alone, but also deals with the study of carbohydrates combining or being associated with other macromolecules - lectins, glycoproteins, glycolipids, enzymes specific for carbohydrates, cell receptors specific for carbohydrates, sugar molecules present in nucleic acids, etc. >> Category: Biotech Research
  • Genome Engineering    By: Rhitu Rai

    This article focuses on new age techniques for editing the genome in vivo which require targeting DNA breaks to defined location followed by either Non homologous end joining or homologous recombination. The origin and basis of TALENs is discussed. >> Category: Biotech Research
  • Role of Beta-glucosidase in Preparing Lactose-Free Pasteurized Milk    By: Hareepriyaw M

    The enzyme lactase or Beta-galactosidase is released by the intestinal villi and it hydrolyzes the lactose into galactose and glucose. Lactase is known to be responsible for the digestion of bovine milk consisting of 4.8% of lactose, on average. Lactose fermentation and malabsorption of lactose in the gut results from the deficiency of the gene responsible for this protein. >> Category: Biotech Research
  • E3 Ubiquitin Ligase Increases Thermal Resistance in Plants    By: Hareepriyaw M

    The increase in global temperatures has shown negative influence on the agricultural production all over the world. Developing thermal resistance in crops has become a great challenge for plant biology researchers and in agriculture. >> Category: Biotech Research
  • Bio-engineering of Buoyant Gas Vesicle Nanoparticles From Haloarchaea    By: Hareepriyaw M

    Gas vesicles are organelles of bacteria and archaea that constitute protein nanoparticles. Gas vesicles are known to provide flotation to the microorganisms, so that oxygen and light are properly available to them especially in phototrophic organisms. Gas vesicles are useful in biotechnological applications as their protein membrane is stable against solubility and they are produced in large scale. The hypotonic lysis of the host can lead to the purified gas vesicles. >> Category: Biotech Research
  • Microbial Biosynthesis of Rare Hexoses    By: Hareepriyaw M

    Monosaccharides and their derivatives are referred to as rare sugars. Monosaccharides have applications in nutrition and pharmaceutical industries and they are used as precursors for drug candidates and natural products. The availability of these rare sugars is restricted and the enzymatic and microbial transformations have become vital tools to synthesize these rare sugars. >> Category: Biotech Research
  • Targeted Disruption of Gene in Bacillus Anthracis    By: Hareepriyaw M

    Bacillus anthracis is a gram+ve bacterium and exists mostly in spore form. The spores will come in contact with the wounds or food of the animals and will enter into the animal. The entered spores will further germinate to a vegetative organism. Most of the infections or 95% of the infections in animals occurs through cutaneous entry while rarely infections occur through pulmonary or gastrointestinal paths. >> Category: Biotech Research
  • Aluminium Resistance in Crop Plants    By: Hareepriyaw M

    Aluminium resistance is found to be governed by genes that belong to two different gene families which encode for transport proteins. Enough data is currently available for increasing aluminium resistance of crop species with the help of biotechnology. This study focuses on the progress made in the research related to aluminium resistance in plants, the genes that are controlling relevant processes and the genetic engineering approaches to increase aluminium resistance in crop plants. >> Category: Biotech Research
  • LecRK-1.9 Enhances Resistance of Solanaceous Plants Against Phytophthora Infestans    By: Hareepriyaw M

    The potato cultivars that are susceptible to Phytophthora infestans were the end products of the potato plants bred for resistance and monitored for the gene called race-specific late blight resistance or R. The R gene belongs to the gene class called nucleotide binding site-leucine rich repeat (NB-LRR). This gene was observed to originate from Mexican wild potato plants like Solanum stoloniferum and S.demissum. >> Category: Biotech Research
  • Production of L-Ornithine by Evolving Cornybacterium Glutamicum    By: Hareepriyaw M

    L-ornithine is an important component of the urea cycle and is a non-essential amino acid. In this study, speE gene was knocked out from the C.glutamicum ATCC 13032 strain deficient in argF and proB genes. The resultant mutant is made to grow in an adaptive environment to evolve metabolically and improve the production of L-ornithine in it. >> Category: Biotech Research
  • Transient Expression of Recombinant Antibodies in HEK-293 Cells    By: Hareepriyaw M

    Human Embryonic Kidney 293 cells or HEK 293 cells, are very easy to grow and transfect very readily and have been widely used in cell biology research for many years. >> Category: Biotech Research

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