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Potential of Stenotrophomonas for Bioremediation of Recalcitrant PollutantsBY: Vipin Chandra Kalia | Category: Environmental-Biotechnology | Submitted: 2016-10-12 01:50:16
Article Summary: "Environmental pollution is caused by heavy use of highly recalcitrant organic compounds -insecticides and pesticides - Classified as priority pollutants by USEPA. Major causes of concern are: (i) Groundwater pollution, (ii) Toxic accumulation in food chain, (iii) Great ecological imbalance, (iv) Carcinogenic nature. Consequently.."
Potential of Stenotrophomonas for Bioremediation of Recalcitrant Pollutants
Authors: Vipin Chandra Kalia
Microbes can do highly diverse things ranging from producing biofuels, biopolymers to carry out bioremediation. Recently, the microbes which are gaining importance as the most effective for biotechnological applications belong to Bacillus, Citrobacter and Stenotrophomonas. Microbial diversity in soils contaminated with polycyclic aromatic hydrocarbons (PAHs), Effluent Treatment Plants (ETPs) treating pesticide and pharmaceutical industrial waste waters, includes: Alcaligenes, Bacillus, Citrobacter, Pseudomonas, Rhodococcus and Stenotrophomonas. Most versatile organisms belonging toStenotrophomonas are able to treat aromatic compounds either individually or in combination with Bacillus, Pseudomonas, Flavimonas, Morganella spp.
Diversity of Stenotrophomonas
Initially these organisms were grouped under Pseudomonas, and Xanthomonas and only more recently have been named asStenotrophomonas. Presently, 8 recognized Stenotrophomonas spp. exist: S. maltophilia, S. nitritireducens,S. acidominiphilia, S. rhizophilia, S. koreensis, S. terrae, S. humi, and S. chelatiphaga. S. dokdonensis has been transferred to Pseudoxanthomonas as P. dokdonensis.
Ecological and metabolic (genetic and functional) diversity of S. maltophilia implies high taxonomic heterogeneity. S. maltophilia shows 4 phylogenetic lineages. S. maltophilia posses 40 genomic islands (3-70 Kb in size), encoding for around 50 proteins. Large islands encode for integrases, etc., which are instrumental in horizontal gene transfer (HGT) from Xanthomonas compestris and X. axonopodis. High diversity among clinical isolates of S. maltophilia is due to polymorphic mutations. Restriction Endonucleases (REs) - DraI and XbaI digestion revealed unique DNA profile in Stenotrophomonas spp. S. maltophilia was distinguished from Xanthomonas through restriction mapping and 16S rRNA gene sequencing.
• Excessive use of nitrogenous fertilizers leads to emission of green house gases such as NO and N2O. S. maltophilia can carry out aerobic denitrification, which reduces nitrate to nitrite.
• S. maltophilia degrades xenobiotics: pesticides, insecticides, dyes, herbicides - atrazine (2-chloro-4-ethylamino-1,3,5 triazine) and 2,4-dichlorophenoxyacetic acid (2,4-D), hexachlorocyclohexane (HCH), detoxify high molecular weight PAHs, cyclotrimethylene trinitroamine derivatives (RDX), oil products. S. maltophilia Ac degrades different substrates, including polyol-L-glucitol, nonylphenol polyethoxylates – the synthetic nonionic surfactants used for textile, leather and paper industries.
• Nylon, Feather degradation and alfatoxins
• Complete degradation of catechol, 4-methylcatechol and hydroquinone within 48 h.
Heavy metal toxicity
S. maltophilia shows resistance to multiple antimicrobial agents, can tolerate a wide range of heavy metals: Cd, Pb, Co, Zn, Hg, Ag, can reduce selenite (50 mM) to selenium (Seo) and tellurite (25 mM) to tellurium (Teo). S. chelatiphaga sp. nov. has an ability to degrade EDTA, can oxidize arsenite As(III) to arsenate As(V). S. maltophilia can be exploited for bioremediation of contaminated ground water, detoxify selenate and arsenate contaminated sites.
Biofilm in bioremediation
Biofilms represent microbial communities, gene expression varies with respect to free floating or planktonic members: antibiotic resistance, aggregation, biofilm formation and expression of virulence factors. Stenotrophomonas produces biofilms even in 100 ng/L [CrVI], can transform Cr(VI) to Cr(III) at 300 mg Cr(VI)/L. S. maltophilia embedded in biofilms could biodegrade dodecylbenzene sulfonates 2 times faster than the free floaters. Polycyclic alkanes are recalcitrant to microbial degradation, however it was found to induce bacterial cell aggregation, which augmented degradation of xenobiotics.
The versatile Stenotrophomonas
It acts as plant growth enhancers, biopesticides and antagonistic against pathogens: (i) S. maltophilia R13 has the potential to act as PGPR bioinoculant as it produces 18 free amino acids, keratinolytic activity and IAA production, (ii) Amenable to exploitation for biocontrol of plant pathogens and act against the human pathogenic fungus Candida albicans. It removes Au(III) from contaminated wastewater through its absorption capacity, can grow in limited nutrient – siderophore and. Its refractile toxic bodies enhance competitiveness
Biotechnological importance: (i) Agriculture - to compete in rhizosphere, (ii) Medicine - by producing antibiotics, and (iii) Industry – enzymes (proteases) - may “revolutionize” detergents.
A cautionary note
Stenotrophomonas are opportunistic pathogen next to Pseudomonas aeruginosa. S. maltophilia causes bacteraemia and pneumonia, endocarditis, respiratory tract infections. S. maltophilia can be serious pathogen in cancer patients. S. maltophilia can also produce macrocyclic lactam antibiotics: (i) alteramid A, and (ii) maltophilin.
Most “dangerous” properties:
· Capacity to resist drugs (as it possess a multidrug efflux pump SmeDEF), heavy metals
· Produces extracellular enzymes – lipases, fibrolysin, proteases and biofilm formation - characteristics of emerging pathogens.
Difficult to develop an optimal therapy against S maltophilia.
1. Agarwala M, Choudhury B, Yadav RNS (2014) Comparative study of antibiofilm activity of copper oxide and iron oxide nanoparticles against multidrug resistant biofilm forming uropathogens. Indian J Microbiol 54:365-368. doi:10.1007/s12088-014-0462-z
2. Alipiah NM, Shamsudin MN, Yusoff FM, Arshad A (2015) Membrane biosynthesis gene disruption in methicillin-resistant Staphylococcus aureus (MRSA) as potential mechanism for reducing antibiotic resistance. Indian J Microbiol 54:41-49. doi:10.1007/s12088-014-0488-2
3. Bhushan A, Joshi J, Shankar P, Kushwah J, Raju SC, Purohit HJ, Kalia VC (2013) Development of genomic tools for the identification of certain Pseudomonas up to species level. Indian J Microbiol 53:253-263. doi: 10.1007/s12088-013-0412-1
4. Gui Z, Wang H, Ding T, Zhu W, Zhuang X, Chu W (2014) Azithromycin reduces the production of α-hemolysin and biofilm formation in Staphylococcus aureus. Indian J Microbiol 54:114-117. doi: 10.1007/s12088-013-0438-4
5. Kalia VC, Kumar R, Kumar P, Koul S (2016) A genome-wide profiling strategy as an aid for searching unique identification biomarkers for Streptococcus. Indian J Microbiol 56:46-58. doi:10.1007/s12088-015-0561-5
6. Kalia VC, Prakash J, Koul S, Ray S (2016) Simple and rapid method for detecting biofilm forming bacteria. Indian J Microbiol 56:1-3. doi: 10.1007/s12088-016-0616-2
7. Kalia VC, Kumar P (2015) Genome wide search for biomarkers to diagnose Yersinia infections. Indian J Microbiol 55:366-374. doi:10.1007/s12088-015-0552-6
8. Kalia VC, Kumar P, Kumar R, Mishra A, Koul S (2015) Genome wide analysis for rapid identification of Vibrio species. Indian J Microbiol 55:375-383. doi:10.1007/s12088-015-0553-5
9. Kalia VC, Kumar P (2015) Potential applications of quorum sensing inhibitors in diverse fields. In: Quorum Sensing vs Quorum Quenching: A Battle with No End in Sight, 359-370. Editor: VC Kalia. Springer India. doi:10.1007/978-81-322-1982-8_29
10. Kaur G, Rajesh S, Princy SA (2015) Plausible drug targets in the Streptococcus mutans quorum sensing pathways to combat dental biofilms and associated risks. Indian J Microbiol 55:349-357. doi:10.1007/s12088-015-0534-8
11. Kekre A, Bhushan A, Kumar P, Kalia VC (2015) Genome wide analysis for searching novel markers to rapidly identify Clostridium strains. Indian J Microbiol 55:250257.doi:10.1007/s12088-015-0535-7
12. Koul S, Kalia VC (2016) Comparative genomics reveals biomarkers to identify Lactobacillus species. Indian J Microbiol 56:253-263. doi:10.1007/s12088-016-0605-5
13. Kumar R, Koul S, Kumar P, Kalia VC (2016) Searching biomarkers in the sequenced genomes of Staphylococcus for their rapid identification. Indian J Microbiol 56:64-71.doi:10.1007/s12088-016-0565-9
14. Moroeanu VI, Vamanu E, Paun G, Neagu E, Ungureanu OR, Eremia SAV, Radu GL, Ionescu R, Pelinescu DR (2015) Probiotic strains influence on infant microbiota in the in vitro colonic fermentation model GIS1. Indian J Microbiol 55:423-429. doi: 10.1007/s12088-015-0542-8
15. Prakasham RS, Kumar BS, Kumar YS, Kumar KP (2014) Production and characterization of protein encapsulated silver nanoparticles by marine isolate Streptomyces parvulus SSNP11. Indian J Microiobiol 54:329-336. doi:10.1007/s12088-014-0452-1
16. Saxena A, Mukherjee M, Kumari R, Singh P, Lal R (2014) Synthetic biology in action: Developing a drug against MDR-TB. Indian J Microbiol 54:369-375. doi:10.1007/s12088-014-0498-0
17. Selvakumaran S, Kapley A, Kashyap SM, Daginawala HF, Kalia VC, Purohit HJ (2011) Diversity of aromatic ring-hydroxylating dioxygenase gene in Citrobacter. Bioresour Technol 102: 4600-4609. doi: 10.1016/j.biortech.2011.01.011
18. Selvakumaran S, Kapley A, Kalia VC, Purohit HJ (2008) Phenotypic and phylogenic groups to evaluate the diversity of Citrobacter isolates from activated biomass of effluent treatment plants. Bioresour Technol 99:1189-1195. doi: 10.1016/j.biortech.2007.02.021
19. Shang Z, Wang H, Zhou S, Chu W (2014) Characterization of N-acyl-homoserine lactones (AHLs)-deficient clinical isolates of Pseudomonas aeruginosa. Indian J Microbiol 54:158-162. doi:10.1007/s12088-014-0449-9
20. Verma V, Raju SC, Kapley A, Kalia VC, Daginawala HF, Purohit HJ (2010) Evaluation of genetic and functional diversity of Stenotrophomonas isolates from diverse effluent treatment plants. Bioresour Technol 101:7744-7753. doi: 10.1016/j.biortech.2010.05.014
21. Verma V, Raju SC, Kapley A, Kalia VC, Kanade GS, Daginawala HF, Purohit HJ (2011) Degradative potential of Stenotrophomonas strain HPC383 having genes homologous to dmp operon. Bioresour Technol 102:3227-3233. doi: 10.1016/j.biortech.2010.11.016
22. Wang R, Fang S, Xiang S, Ling S, Yuan J, Wang S (2014) Generation and characterization of a scFv antibody against T3SS needle of Vibrio parahaemolyticus. Indian J Microbiol 54:143-150. doi: 10.1007/s12088-013-0428-6
23. Yu S, Peng Y, Zheng Y, Chen W (2015) Comparative genome analysis of Lactobacillus casei: Insights into genomic diversification for niche expansion. Indian J Microbiol 55:102-107. doi: 10.1007/s12088-014-0496-2.
About Author / Additional Info:
Researcher at Microbial Biotechnology and Genomics at CSIR-IGIB, Delhi.
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