Publish Your Research Online
Get Recognition - International Audience
Request for an Author Account | Login | Submit Article
|HOME||FAQ||TOP AUTHORS||FORUMS||PUBLISH ARTICLE|
Edible Vaccines: For Pharmaceutical ProductsBY: Kumatkar Raghunath Bhimrao | Category: Genetics | Submitted: 2016-10-21 02:04:59
Article Summary: "Vaccines that one can eat, called edible vaccines are among the most unusual approaches for administering new vaccines..."
Edible vaccines: for pharmaceutical products
Authors: Kumatkar Raghunath Bhimrao, Vivek K Singh, and Pratik I Bhoyar.
CCS Haryana Agricultural University, Hisar, Haryana, 125004.
Vaccines that one can eat, called edible vaccines are among the most unusual approaches for administering new vaccines. Edible vaccines are like subunit preparation, in that they are engineered to contain antigen, but bear no genes that would enable whole pathogen to form. These vaccines are mucosal-targeted vaccines, which cause stimulation of both systematic and mucosal immune response. Edible vaccines are currently being developed for a number of human and animal diseases, including measles, cholera, foot and mouse disease, and hepatitis B and C. Many of these diseases require booster vaccination or multiple antigens to induce and maintain protective immunity (Gidding et al., 2000). Plants have capacity to express more than one transgene, allowing delivery of multiple antigens for repeated inoculations (Conrad &Fiedler, 1998).
Why do we need to use Plant Vaccines:
Plant systems do not harbor human or animal pathogens and, therefore, they do not transmit such pathogens along with the target subunit vaccine (Schillberg et al., 2005). Moreover, they cost less to produce than via fermentation or bioreactors; plants can be grown in the field or in a greenhouse relatively inexpensively. When produced in edible parts of the plant, such as grain, fruit or even leaves, subunit vaccines may not require purification. Maintaining the antigenic protein within plant cells that are edible may also contribute to stability and reduce degradation. Another advantage of producing subunit vaccines in edible parts of a plant is the potential to deliver the orally rather than intramuscularly, providing a simple and easy means of administration to humans and animals (Mishra et al., 2008).
Mechanism of Action
Oral delivery stimulates mucosal immunity in the tissues lining the respiratory system and eliminates injection-related hazards. Plants structure may help in maintaining the antigenic property even after degradation in intestine. The antigens in transgenic plants are delivered through the tough outer wall of plant cells, which protects them from gastric secretions and finally break up in the intestines. The antigens are released and uptake by M cells in the intestinal lining that overlie peyer's patches and gut-associated lymphoid tissue (GALT), passed on to macrophages, other antigen-presenting cells; and local lymphocyte populations, generating serum IgG, IgE responses, local IgA response and memory cells, which would promptly neutralize the attack by the real infectious agent.
Achievements: a number of plants vaccines have been developed in many crops/plants like; transgenic potato expressing Norwalk virus antigen showed seroconversion, Cholera virus (CT-B gene) & hepatitis B in Banana, potato and vaccine for Newcastle disease in tobacco.
Advantages of Edible Vaccine:
· Development of immunotolerance to vaccine peptide or protein.
· Consistency of dosage from fruit to fruit, plant to plant, and generation to generation is not similar.
· Stability of vaccine in fruit is not known.
· Selection of best plant is difficult.
More research is being done to:
ü Determine if one vaccine can help protect against multiple diseases.
ü Determine what would be a good dose or how often vaccine would need to be taken.
ü If vaccine can cause a negative response instead of positive.
Schillberg S, Twyman RM, Fischer R (2005). Opportunities for recombinant antigen and antibody expression in transgenic plants - technology assessment. Vaccine 23: 1764-1769.
Mishra N, Gupta PN, Khatri K, Goyal AK, Vyas SP (2008). Edible vaccines: A new approach to oral immunization. Indian Journal of Biotechnology 7: 283-294.
Giddings, G., Allison, G., Brooks, D. and Carter, A. (2000). Transgenic plants as factories for biopharmaceuticals. Nature Biotechnology 18, 1151-5.
Conrad, U and Fiedler, U (1998). Compartment-specific accumulation of recombinant immunoglobulins in plant cells: an essential tool for antibody production and immunomodulation of physiological functions and pathogen activity.
Plant Molecular Biology 38, 101-9.
About Author / Additional Info:
I am currently pursuing Ph.D. from CCS HAU, Hisar
Comments on this article: (0 comments so far)
• Lettuce Cultivation in India
• Three Phase Protein Purification Strategies
• Biotechnology and Environment Protection
• Joule Unlimited - Pioneering Alternate Fuel and its Advantages
Latest Articles in "Genetics" category:
• The Science and History of Genetics. How It Predicts the Genetic Code
• Telomeres: Is It Responsible For Ageing and Cancer?
• Human Genetic Engineering,its Methods and Ethics
• Gene Mutation And Cancer
• DNA Technology Used in Forensics
• DNA Fingerprinting: Uses and Methods Involved
• Treatment of Genetic Diseases by Gene Therapy
• Human Intelligence and Genetics
• Ethical Issues Related to Human and Animal Cloning
• Mitochondrial DNA and Forensic
• DNA Footprinting and Gene Sequencing
• Biotechnology and Types of Cloning
• Designer Babies:Method and Ethical Issues
• Prenatal Diagnosis: Non-invasive and Invasive Techniques
• What are the Benefits of Genetic Engineering?
• The Advantages and Disadvantages of Genetic Engineering in Humans
• Types of Genetic Disorders
• Bovine Somatotropin: A Growth Hormone
• Advantages and Disadvantages of Genetically Modified Food
Important Disclaimer: All articles on this website are for general information only and is not a professional or experts advice. We do not own any responsibility for correctness or authenticity of the information presented in this article, or any loss or injury resulting from it. We do not endorse these articles, we are neither affiliated with the authors of these articles nor responsible for their content. Please see our disclaimer section for complete terms.
Copyright © 2010 biotecharticles.com - Do not copy articles from this website.
ARTICLE CATEGORIES :
| Disclaimer/Privacy/TOS | Submission Guidelines | Contact Us