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Microsatellite Marker and its Utility [PDF]

BY: Prasenjit Debnath | Category: Genetics | Submitted: 2017-04-02 09:13:18
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Article Summary: "The article gives an overview of the basics of micro-satellite and its development; its types and how can it be used for the purpose of crop improvement..."

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Authors: 1Prasenjit, D., 2Anirudha, S. K. and 3Mallar, N.K.
1,2 M.Sc.(Agri.), Dept. of Biotechnology, UAS, Dharwad, Karnataka
3 M. Sc (Agri.), Dept. Of Biotechnology, AAU, Jorhat
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A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2-5 base pairs) are repeated, typically 5-50 times. Microsatellites are used in population genetics to measure levels of relatedness between subspecies groups and individuals.

• The term microsatellite was first coined by Litt and Luty (1989) .
• Microsatellites are simple repeats.
• Motifs consisting of 1 to 6 base pairs.
• The mutation rate of this type of genetic marker has been estimated to be between 10−2 and 10−4 per generation.
• They are inherited in a Mendelian fashion as codominant markers. This microsatellite markers co-segregate in to the offsprings during F1 development . So, we can use these markers to screen the F1 plants.
• Flanking region is highly conserved in related species.

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About Author / Additional Info:
M.Sc.(Agri.) in Molecular Biology and Biotechnology, IABT, UAS, Dharwad.
Research Area: Molecular Breeding.

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