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Plant Bioreactors Types - Their Advantages and Disadvantages

BY: Padma Kumar | Category: Others | Submitted: 2010-07-16 06:16:50
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Article Summary: "A discussion on the different plant bioreactor expression systems with examples. A look at Seed-based, Hairy Root and Chloroplast plant bioreactors..."


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Plant bioreactors refer to the use of transgenic plants and cell cultures of plants to make unlimited quantities of commercially important substances like recombinant proteins including antibodies and vaccines using biotechnology oriented techniques.

Most of the research has been directed towards using plant bioreactors to make the following

• Therapeutic proteins
• Edible vaccines
• Antibodies for immunotherapy

Using genetic engineering, cereal plants, fruits plants, legumes and vegetable plants have the capacity to become low cost bioreactors to make molecules that in the normal scheme of things would not have been available from plants. Human growth hormone was the first drug that was produced using plant bioreactors, in this case from the transgenic tobacco.

We will now discuss the different plant bioreactor expression systems especially with reference to where the protein compartmentalizes in these bioreactors.


Seed-based plant bioreactors

Plant seeds accumulate large amount of proteins during their development stage and therefore plant bioreactors based on seed platforms are reckoned as suitable for storing recombinant proteins.

An example is the successful expression of the human lysosomal enzyme alpha-L-iduronidase in Arabidopsis thaliana seeds. In seed bioreactors the expression of recombinant proteins is controlled using seed specific promoters like for example in maize globulin-1, and in rice glutelin promoter Gt-1. The advantage of these systems is that, proteins do not degrade at ambient temperature and are stable for long term storage.

However factors such as specificity of expression and subcellular storage environment would decide how specifically seeds could be used for producing desired molecules.

Seed Protein Storage Vacuole Bioreactors

In seed bioreactors, protein storage vacuoles comprising the sub compartments namely, matrix, globoid and crystalloid are dominant compartments for storing recombinant proteins. The matrix is suitable for soluble storage proteins, globoid for hydrolytic enzyme, and crystalloid for BP-80 TMD and the CT of alpha-tonoplast intrinsic protein sequences.


Seed Oil Body Bioreactors

Seed oil body is encircled by the protein oleosin which are ideal carriers for heterologous protein production and also provide a recognition signal for lipase binding during oil mobilization in seedlings. Seed oil body is bioreactors that can store large amount of macromolecules.

An example is the expression of fusion protein containing oleosin and the β-glucuronidase in seed oil body. Another example is the manufacture of the anticoagulant hirudin in the oil body of seeds Brassica napus and Brassica carinata

Plant Suspension Cultures

They are used to express recombinant proteins, secondary metabolites and antibodies transported to subcellular organelles. For example, is the expression of 80-kDa human lysosomal protein (controlled by 3 5S CaMV and a signal peptide) in transgenic tobacco BY-2 cells culture media.

Hairy Root System Bioreactor

The Hairy Root System with its rhizosecretion is due to infection of the soil bacterium-Agrobaterium rhizogenes. It offers extreme biosynthetic stability and is suitable for making biopharmaceuticals as for example scopolamine in Hyoscyamus muticus L. hairy root culture

Chloroplast bioreactor

Insulin, interferons and other biopharmaceutical proteins can be made using Chloroplast bioreactor. One method is foreign genes are inserted into nuclear chromosomes and with peptides target expressed proteins into chloroplast. An example is the high yield in the expression of human serum albumin protein in chloroplast.


Comparitive study of plant bioreactor systems

Cost wise, seed, hairy root, cultured cell suspension, oil body and chloroplast bioreactor systems are on the cheaper side to set-up and in terms of product stability seed and oil body systems offer the best prospects. In terms of scale-up capacity seed, oil body and chloroplast bioreactor systems are most suitable.

Advantages and Disadvantages

Generally the practice has been to use cloned animal cells to make antibodies for use as drugs. But there is always the remote chance of unwanted allergic reactions due to antibodies of animal origin, and that apart, contamination of the antibody product due to proteins and viruses of animal origin is a distinct possibility. Such problems do not arise when plants are used to make antibodies because plants do not generally serve as hosts for human and animal pathogens

Making recombinant proteins in transgenic plants is relatively cheap as compared to the cost of running fermentors although extraction and purification processes have to be efficient. For example these proteins are expressed in the plant seeds which all look similar and the recombinant protein has to be cleaved enzymatically or by other means.
But these seeds can be stored and processed when required unlike animal cell production methods which require immediate purification.

Conclusion

More and more uses of plant bioreactors are coming up these days. For example, plant bioreactors are being investigated for making enzymes suitable for use in feed additives and in food. Another use of plants is to make genetically engineered plants that can produce seeds which can function as a delivery mechanism for various industrial enzymes

As you can see these processes go far beyond the application of biotechnology in traditional agriculture, and so today, transgenic plants can produce on a mass scale proteins for agricultural, veterinary and pharmaceutical use. These processes are mainly carried out by cost conscious biopharma and enzyme companies.

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Comments on this article: (4 comments so far)

Comment By Comment
ashakiran
2011-11-19 09:03:33 239
any information about who manufacturer bioreactors will be help ful
Padma Kumar - Author
2011-12-03 11:30:37 242
Sorry for the late reply. xcellerex makes bioreactors Nanologix has patent for hydrogen bioreactors---that aim to use wastewater to make hydrogen. Greenfuel technologies had patent for making algae bioreactors, but they closed shop. Other plant based bioreactors are mostly patented technologies and not mass produced. I hope I could answer your question.
Eddie
2016-02-03 07:02:32 1035
I am currently experimenting wih cell tissue culture as a hobby and am looking to design a high-end bioreactor. As a layman, I find it difficult to select an optimal design in order to supercharge growth quality of my plants. I have been looking into atomized aeroponic systems as I use them for my rooted plants. Is this the most efficient method for the creation of high quality plant clones? If so, should the atomized pressurized fog be direct or indirect?
Padmakumar
2016-02-09 22:38:44 1037
Hi Eddie, I do not have any practical exposure to atomized aeroponic systems and hence cannot accurately answer your question. As regards whether a.a. systems can create high quality plant clones?----may be you should talk to people already running sustainable plant factories.

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