Biotech Articles
Publish Your Articles Online
Get Recognition - International Audience

Request for an Author Account   |   Login   |   Submit Article
 
 
HOME FAQ TOP AUTHORS FORUMS PUBLISH ARTICLE
 
 

Plant and Animal Tissue Culture: Procedure, Benefits and Limitations

BY: bluemoon silverstars | Category: Others | Submitted: 2010-05-27 21:57:02
       No Photo
Article Summary: "Plant and animal tissue culture are of great importance. they can be grown to enhance the production of the plants and animals. Plant tissue culture has many benefits but animal cell and tissue culture has some limitations..."


Share with Facebook Share with Linkedin Share with Twitter Share with Pinterest Email this article
     


Tissue culture is defined as the growth of cells and tissues of the organism out of its body. Many mediums are required to accomplish the task for example semi-solid, liquid and growth medium. Broth agar is considered as best for tissue and cell culture. It consists of nutrients, sugars, vitamins and hormones. These components in the gel make the plant to grow rapidly and produce new plantlets. Plant tissue culture is also a technique used for plants.

Historical background of tissue culture is that in 1885, Wilhelm Roux extracted small part of the medullary plate from the embryo of a chicken and cultured it. For several days he put this culture under observation and established the basis of tissue culture. Then in 1907, Ross Granville Harrison prepared a medium of clotted lymph and placed frog nerve cells in it. He observed the growth of the nerve cells in that medium. In plants tissue culture is used to increase the growth of the plants. The laboratory atmosphere is best suited for the plant tissue culture, because it s sterile and free of germs.

Steps involved in Tissue culture:-
1) First a stem cell or small part of a leave is taken which is also called as an explant. This expalnt is placed in the container of the tissue culture. Before placing the explant in the tissue culture, tissue culture should be sterilized so that no bacteria or fungus can attack the plant.
2) The tissue culture is then placed in the agar gel which is rich with nutrients, sugars and other hormones to enhance the growth of the explant.
3) Two things can occur in the tissue culture. Either the explant will start making big blobs which are called as callus or it will start producing new shoots directly from the tissue of the explant.
4) When new plantlets are produced, some of them can be removed from the tissue culture and can be placed in a new tissue culture. This way, in a limited time thousands of plants will produce.
5) Plantlet will be removed from the culture and will be transferred to the soil and they will start growing like any other traditionally growing plants.
6) Using this technique of tissue culture, laboratory plants will also be able to grow normally.

Plant tissue culture is beneficial agriculturally. By using the techniques of tissue culture, many thousands of plants can be produced in a very short time. This technique is also useful in the sense, that by using only a single parent plant, many plants can be produced. As all the procedures of tissue culture take place in the sterile environment that is why it is difficult for the germs or insects to attack the crops and growth can be increased. Plant tissue culture can also be used at cellular level. Agriculturists can observe the cells of the plants and make certain changes in the genome to make them herbicide resistant.

Like plants, animal cells can also culture. Following step take place in animal tissue culture:
1) From a particular specimen, animal cell is extracted.
2) A special kind of medium is required for the animal tissue culture. Animal cell or tissue is placed in the medium under aseptic environment.
3) For the animal tissue culture, specific temperature is required and cells are grown in the culture. For example, the temperature required for the human cells to culture is 37 degrees which is the normal body temperature.
4) Carbon dioxide is also required.

There is a difference of animal cells culture than plants tissue culture. Undergoing the limited number of cell cycles, animal cells begin to degrade. When the animal cell culture is produced in the medium, there is a lot of need of changing the medium otherwise animal cells will not grow properly. There is difficulty of growing the animal cell and tissue culture because unlike plants which need nutrients and other components, animal cells need vitamins, proteins and other body components which are slightly difficult to provide to the medium.

About Author / Additional Info:


Search this site & forums
Share this article with friends:



Share with Facebook Share with Linkedin Share with Twitter Share with Pinterest Email this article

More Social Bookmarks (Digg etc..)


Comments on this article: (5 comments so far)

Comment By Comment
tejpal sharma
2010-11-13 19:40:12 91
thanks,for this help .your langauge is so simple .i like this
bluemoon silverstars - Author
2010-12-08 03:47:48 106
I keep my language simple so readers can understand the article. Thanks!
jancy
2011-02-11 08:59:04 125
i can easily understand this article. thanks
dhanak siddiqui
2011-03-12 13:36:54 142
yes all above statements are true,the language is sooooooo simple.i like that
hajar
2011-03-16 02:32:13 143
thanks a lot, i've being looking for this

Leave a Comment   |   Article Views: 16576



Additional Articles:
•   Laboratory Data For Blood Test
•   Various Methods for Quantitation of Proteins
•   Cellphone Radiation - Health Effects and Means of Protection
•   Seed Storage Proteins

Latest Articles in "Others" category:
•   Biotechnology, Its Techniques and Human Health
•   Techniques of Biotechnology
•   Nanomedicine and Disease Treatment
•   Biotechnology and Livestock
•   Bioinformatics: Combination of Biotechnology and Information Technology
•   Gene Patenting and Its Uses
•   Polymerase Chain Reaction: A Technique of Biotechnology
•   Pharmacogenomics: Benefits and Barriers
•   Human Genome Project: Ethical and Legal Issues
•   Therapeutics and Biotechnology
•   Biotechnology: A Revolutionary Field and Biotech Challenges
•   Recombinant DNA Technology
•   Environment and Biotechnology
•   Biosensors: Role in Biotechnology
•   Human Insulin and Recombinant DNA Technology
•   Biotechnology and Its Applications
•   Genetic Engineering and its Methods
•   Types of Gene Mutations - Diseases Caused By Gene Mutation
•   Applications of Enzymes in Biotechnology


Important Disclaimer: All articles on this website are for general information only and is not a professional or experts advice. We do not own any responsibility for correctness or authenticity of the information presented in this article, or any loss or injury resulting from it. We do not endorse these articles, we are neither affiliated with the authors of these articles nor responsible for their content. Please see our disclaimer section for complete terms.
Page copy protected against web site content infringement by Copyscape
Copyright © 2010 biotecharticles.com - Do not copy articles from this website.

ARTICLE CATEGORIES : Agriculture   |   Applications   |   Bioinformatics   |   Biotech Products   |   Biotech Research   |   Biology   |   Careers   |   College / Education   |   DNA   |   Environmental Biotech   |   Genetics   |   Healthcare   |   Industry News   |   Issues   |   Nanotechnology   |   Others   |   Stem Cells   |   Press Release   |   Toxicology  

| Disclaimer/Privacy/TOS | Submission Guidelines | Contact Us