Publish Your Research Online
Get Recognition - International Audience
Request for an Author Account | Login | Submit Article
|HOME||FAQ||TOP AUTHORS||FORUMS||PUBLISH ARTICLE|
Recombinant DNA TechnologyBY: bluemoon silverstars | Category: Others | Submitted: 2010-05-30 21:57:23
Article Summary: "Recombinant DNA technology uses to construct a DNA segment from two different organisms. The gene of interest is inserted in the vectors and vectors are further used for certain purposes after cloning..."
Recombinant DNA technology is used to produce DNA segments after different processes joined together. To make a recombinant DNA, different DNA segments are taken and one recombinant DNA molecule is produced. When the recombinant DNA is produced, DNA segment are taken from two different species, due to which new genetic combination is produced which is useful for agriculture, medicine and other fields of science. Like cloning technique, this technique is also used in a woman who is infertile. When this technique is used, new hormones are produced, which have the same biology as the ones from which they are produced.
Recombinant DNA technique got recognition by scientist Peter Lobban. They isolated the DNA segments from the two different species and joined them together and make a recombinant DNA. This recombinant DNA can be inserted in the host cells with already existing DNA to alter the different traits or characteristics in the genes. This technique is different from cloning in the sense that in cloning, identical organism is produced by using the genetic makeup of the organism but in recombinant technique, DNAs are taken from two different organisms. Recombinant is present in the form of a vector and is used in this form further. This vector can be inserted in the plasmid and are used fro medicinal purposes.
Steps Involved in Recombinant DNA technology:-
Before going into the detail of the technique, first have look t the vectors and plasmids. Vectors are the piece of the DNA molecule, which have the ability to replicate independently. Commonly used vectors for the recombinant DNA technology are the plasmids and viral phages. Plasmids are the extra chromosomal structures.
1) A gene is extracted from the DNA molecule and is inserted into the vector.
2) The foreign gene is inserted into the plasmid and is called as recombinant DNA.
3) The vector is then cloned. It is useful to get multiple copies of the vector for the ease of the experiment that if one vector does not work, others can be used for the purpose. Then it is inserted in the host cell to express the proteins.
4) Multiple copies of vectors now can be inserted in different host cells. For example yeast cells, mammalian cells or bacterial cells can use these vectors.
5) The host cell once gets the foreign vector; it will start synthesizing the foreign protein from the recombinant DNA.
6) When we get the recombinant proteins in excess amount, they can be isolated from the host cells and can be purified.
Benefits of Recombinant DNA Technology:-
Recombinant DNA technique is very much useful for detecting and diagnosing the diseases and helps in treating them. Many genetic diseases can be detected with this technique. This technique has also helped in the formation of insulin, growth hormones and other proteins. People can be detected with different types of cancers like breast cancer with this technique. Mutation is the major cause of genetic diseases. Recombinant DNA technology makes it possible to detect mutations in the gene sequences and making corrections in them. Mapping of genes or transfer of genes from one organism to the other is all made possible by Recombinant DNA technology.
About Author / Additional Info:
Comments on this article: (0 comments so far)
• Clean Rooms Nomenclature - Class 100, Class 1000 Areas.
• Virtual Screening- a Promising Approach to Drug Discovery
• New Dimension of Scope and Career in Microbiology
• Ice-minus Bacteria ( P. Syringae) - Frost-fighting Superman?
Latest Articles in "Others" category:
• Biotechnology, Its Techniques and Human Health
• Techniques of Biotechnology
• Nanomedicine and Disease Treatment
• Biotechnology and Livestock
• Bioinformatics: Combination of Biotechnology and Information Technology
• Gene Patenting and Its Uses
• Polymerase Chain Reaction: A Technique of Biotechnology
• Pharmacogenomics: Benefits and Barriers
• Human Genome Project: Ethical and Legal Issues
• Plant and Animal Tissue Culture: Procedure, Benefits and Limitations
• Therapeutics and Biotechnology
• Biotechnology: A Revolutionary Field and Biotech Challenges
• Environment and Biotechnology
• Biosensors: Role in Biotechnology
• Human Insulin and Recombinant DNA Technology
• Biotechnology and Its Applications
• Genetic Engineering and its Methods
• Types of Gene Mutations - Diseases Caused By Gene Mutation
• Applications of Enzymes in Biotechnology
Important Disclaimer: All articles on this website are for general information only and is not a professional or experts advice. We do not own any responsibility for correctness or authenticity of the information presented in this article, or any loss or injury resulting from it. We do not endorse these articles, we are neither affiliated with the authors of these articles nor responsible for their content. Please see our disclaimer section for complete terms.
Copyright © 2010 biotecharticles.com - Do not copy articles from this website.
ARTICLE CATEGORIES :
| Disclaimer/Privacy/TOS | Submission Guidelines | Contact Us