Publish Your Research Online
Get Recognition - International Audience
Request for an Author Account | Login | Submit Article
|HOME||FAQ||TOP AUTHORS||FORUMS||PUBLISH ARTICLE|
Epigentek Introduces a Cost-Effective and Reliable Means to Validate Antibodies for ChIPBY: Natalie Crowley | Category: Press-Release | Submitted: 2014-06-13 22:50:34
Article Summary: "The newly released Pre-Sure ChIP Antibody Validation Kit is a fast, affordable, and robust method for ChIP-grade validation of antibodies for use in ChIP-PCR, ChIP-on-chip, and ChIP-seq..."
Epigentek today announced the debut of Pre-Sure(TM), a commercialized kit for quickly validating and qualifying whether antibodies are suitable for use in chromatin immunoprecipitation (ChIP). Officially known as the Pre-Sure(TM) ChIP Antibody Validation Kit, the innovative product is part of the company's effort to improve the integrity of ChIP-grade antibodies in the marketplace as well as to provide a fast and affordable means for researchers to validate their own antibodies for ChIP use.
Chromatin immunoprecipitation is a major lab technique for determining whether specific proteins are associated with certain genomic regions, and is highly useful for investigating histone modifications and various epigenetic mechanisms through ChIP-PCR, ChIP-on-chip, or ChIP-seq. The success of the technique is highly dependent on an antibody's ability to capture protein/DNA complexes specifically and sensitively, which makes it important for researchers to identify suitable antibodies as "ChIP-grade". The largest bottleneck to a reliable ChIP experiment is the high variability of antibodies available on the market, which Epigentek addresses with Pre-Sure(TM).
"The market is saturated with questionable ChIP-grade claims of antibodies due to improper use of Western blot and immunohistochemistry data as the validation basis, both of which are actually unreliable applications for ChIP determination," said William Lee, vice president of operations at Epigentek. "This problem is compounded by the lack of a cost-effective and easy validation method for researchers."
Epigentek's method solves the problem by providing both researchers and vendors the ability to validate antibodies with an inexpensive, fast, and proper method without having to do a full blown and cumbersome ChIP-PCR or ChIP sequencing run. In addition, the low cost and labor aspects of Pre-Sure(TM) provide antibody manufacturers an opportunity to economically screen their antibodies for ChIP, potentially also improving market pricing for researchers. The estimated cost, including labor, to test each antibody via Pre-Sure(TM) is less than $15, whereas validation by ChIP-PCR and ChIP-seq are in excess of $100 and $1,500 per antibody, respectively.
The 96-well plate based Pre-Sure(TM) kit utilizes control antibodies and a fluorescent method to deliver a "ChIP-Grade Intensity" reading of the test antibodies against input chromatin. In less than 3 hours, one could test up to 43 antibodies simultaneously using this kit and a fluorescence-based microplate reader.
Back in 2006, Epigentek was the first company to offer a microplate-based ChIP method to resolve throughput bottlenecks, and the Pre-Sure(TM) ChIP Antibody Validation Kit underscores another milestone of innovative efforts to overcome research obstacles in epigenetics.
About Epigentek Group Inc.
Epigentek is a New York-based biotechnology company that focuses on developing and providing innovative technologies, services, and products as a complete and systematic solution for epigenetic related research and drug discovery. Visit the informative website at http://www.epigentek.com.
References (if any)
1. Company Source: http://www.epigentek.com/catalog/news.php?article=51
2. Product Page: http://www.epigentek.com/catalog/pre-sure-chip-antibody-validation-kit-p-3797.html
About Author / Additional Info:
Epigentek is a New York-based biotechnology company that focuses on developing and providing innovative technologies, services, and products as a complete and systematic solution for epigenetic related research and drug discovery. Visit the informative website at http://www.epigentek.com .
Comments on this article: (0 comments so far)
• Biofilms: Their Role in Agriculture
• Biological Weapons - Types, Current Usage Status and Future
• General Inroduction on Biological Sources of Proteases
• Dark Side of Respiratory Chain: Superoxide Formation | Aging & Cell Death
Latest Articles in "Press-Release" category:
• Publish Your Biotechnology Press Releases on BiotechArticles.com
• Ginger Technology (Cures for all diseases)
• International Stem Cell Corporation Announces the Commercial Launch of New Skin Care Products
• Greiner Bio-One in Monroe, NC Opens New Warehouse Facility
• MMS Hosts 2011 Scientific Symposium
• Treatment of Domestic Waste Water Using Natural Horse and Cow Dung
• Genotypic and NIPGR Launch Chickpea Gene Expression Microarray
• Low Fat Diet to Reduce Risk of Breast Cancer.
Important Disclaimer: All articles on this website are for general information only and is not a professional or experts advice. We do not own any responsibility for correctness or authenticity of the information presented in this article, or any loss or injury resulting from it. We do not endorse these articles, we are neither affiliated with the authors of these articles nor responsible for their content. Please see our disclaimer section for complete terms.
Copyright © 2010 biotecharticles.com - Do not copy articles from this website.
ARTICLE CATEGORIES :
| Disclaimer/Privacy/TOS | Submission Guidelines | Contact Us