Authors: Bharat H. Gawade and Zakaullah Khan
Division of Plant Quarantine
ICAR-National Bureau of Plant Genetic Resources
Pusa Campus, New Delhi-110012
Phytoalexins are low molecular weight secondary metabolites with antimicrobial activity, which are synthesized in plants with response to pest/pathogen attack. These are the natural products secreted by plants, accumulates temporarily and have inhibitory effect against bacteria, fungi, nematodes, insects etc. Among phytoalexins, pisatin was the first chemically characterized phytoalexin from pea plants after which large number of phytoalexins were isolated and reported from various crops such as beans, rice, barley, banana etc. (Braga et al. 1991). It able the plants to respond nematode infection by production of antibiotic compounds to limit further spread and development of nematode (Veech, 1982). The word phytoalexin was derived from two greek words- phyto means plant and alexin means warding off compound. The phytoalexin concept was first given by Muller and Borger in 1941 during observation of potato tubers infected with incompatible race of Phytophthora infestans which was capable of induction of hypersensitive response (HR) in potato. They found that the phtyoalexin produced with response to this race significantly reduced the infection by another compatible race of P. infestans.
General properties of phytoalexins:
- These are heterogeneous group of compounds with biological activity against pathogens
- These are considered as molecular markers of disease resistance and often associated with hypersensitive response (HR)
- Production of phytoalexins is elicited by nematode infection in resistant plants
- They are produced in plants after few hours on infection and continue till few days
- Phytoalexins are reported from plant families like leguminosae/fabaceae, solanaceae, brassicaceae/cruciferae
- Phytoalexins are induced HR in cells surrounding the feeding cells of nematodes
|Name of phytoalexin||Crop/plant||Produced in response to||Reference|
|Coumestrol Psoralidin||Phaseolus lunatus||Pratylenchus scribneri||Rich et al., 1977|
|Glyceollin||Glycine max||Meloidogyne incognita||Kaplan et al., 1980|
|Glyceollin I||Glycine max||Heterodera glycines||Huang and Barker, 1991|
|Gossypol (Terpenoid Aldehydes)||Gossypium hirsutum||M. incognita||Veech, 1982|
|Phaseolin||Phaseolus vulgaris||Pratylenchus penetrans||Abawi et al., 1971|
|Medicarpin||Medicago sativa||P. penetrans||Baldridge et al., 1998|
|Phenyl-phenalenones: anigorufone||Musa spp.||Radopholus similis||Holscher et al., 2014|
The above reports suggest that phytoalexins are not extensively studied for nematode resistance in plants but these can have potential in nematode management as in other pathogens.
- Abawi, G.S., Van Etten, H. D., and Mai. W. F. 1971. Phaseollin production induced by Pratylenchus penetrans inPhaseolus vulgaris. Journal of Nematology, 3:301.
- Baldridge, G. D., O’Neill, N. R. and Samac D. A. 1998. Alfalfa (Medicago sativa L.) resistance to the root-lesion nematode, Pratylenchus penetrans: defense-response gene mRNA and isoﬂavonoid phytoalexin levels in roots. Plant Molecular Biology, 38: 999-1010.
- Braga, M.R. et al. 1991. Phytoalexins induction in Rubiaceae. Journal of Chemical Ecology, 17:1079-1090.
- Holscher et al. 2014. Phenalenone-type phytoalexins mediate resistance of banana plants (Musa spp.) to the burrowing nematode Radopholus similis. PNAS, 111.1: 105–110.
- Huang, J. and Barker, K.R. 1991. Glyceollin I in soybean-cyst nematode interactions- spatial and temporal distribution in roots of resistant and susceptible soybeans. Plant Physiology, 96: 1302-1307.
- Kaplan, D. T., Keen, N. T. and Thomason, I. J. 1980. Studies on the mode of action of glyceollin in soybean incompatibility to the root-knot nematode, Meloidogyne incognita. Physiological Plant Pathology, 16: 319-325.
- Rich, J. R., Keen, N. T. and Thomason, I. J. 1977. Association of coumestans with the hypersensitivity of lima bean roots toPratylenchus scribneri. Physiological Plant Pathology, 10: 105-I16.
- Veech, J. A. 1982. Phytoalexins and their role in the resistance of plants to nematodes. Journal of Nematology ,14(1): 149-161.
About Author / Additional Info:
Division of Plant Quarantine, ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi-110012