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RNA Isolation Protocol from Recalcitrant Tissues of Mango [PDF]

BY: Dr. R. R.Sharma | Category: Applications | Submitted: 2017-08-03 05:49:08
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Article Summary: "Ribonucleic acid (RNA) isolation is a critical step in the molecular experiments. Mango is one of the most complex crops from which the RNA isolation is very difficult due to significant difference in the chemical composition of various tissues. Various protocols have been tried but most of them have failed. We attempted to deve.."

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Rapid and Efficient RNA Isolation Protocol from Various Recalcitrant Tissues of Mango (Mangifera indica L.)

Authors: R.R. Sharma and S. V. R. Reddy
Division of Food Science and Postharvest Technology,
ICAR-Indian Agricultural Research Institute, New Delhi - 110 012, India

Mango (Mangifera indica L.) is an economically important fruit crop of India. Based on its popularity in the masses, wide adaptability, high nutritive value, varietal diversity, delicious taste, excellent flavor and attractive appearance, it is appropriately titled as 'King of fruits' in India. It is grown in 2.5 million hectares with a production of 18 million tones ranking first in area and second in production among all other fruit crops cultivated in the country. Mango fruits are universally consumed as fresh fruits and the biochemical changes that occur during ripening impart fruit quality in terms of softening, carotenoid accumulation, and flavor volatile production. All these biochemical events are regulated at gene level and understanding these events is of utmost importance in improving the fruit quality and storage potential, which necessitates isolation of good quality RNA from the mango fruits during different stages of development and ripening.

Size: 543KB

About Author / Additional Info:
Working on production and postharvest management of fruits and vegetables for the last 32 years. I have published more that 130 research articles in journals of international repute. Authored 12 books, 250 popular articles and received several awards and honours of ICAR and Govt. of India

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