Authors: Dr. Sandhya, Dr. Navin Chandra Gupta and Anshika Tyagi
ICAR - National Research Centre on Plant Biotechnology, New Delhi - 110012.
X-ray Crystallography is a scientific method used to determine how the atoms are arranged in a crystalline solid in three dimensional spaces. X- rays are used in the crystallography has wavelength of 0.02 to 100 A. In X-ray crystallography the crystalline atom cause diffraction of incident X-rays in to different path. This article highlights the overview and application of X-ray crystallography in brief.
What is X-ray Crystallography? X-ray crystallography is scientific technique that relies on the diffracted X-rays passing on crystals. It is not an imaging technique. On the basis of diffraction pattern obtained from X-ray scattering off the periodic assembly of molecules or atoms in the crystal, the electron density can be restructure. Using electron density, the mean positions of the atoms in the crystal, their bonding nature and other information can be determined. The goniometer is used for positioning of the crystal.
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