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Most Recent Biotech Research Articles.
  • Targeted Genome Editing in Plants    By: Dr. Monika Dalal

    The ability to incorporate useful genes and to modify genes in the genomes has been the aim of researchers worldwide. The nuclease based techniques of targeted genome editing have emerged as an efficient tool for precise manipulation of gene sequences in their natural chromosomal context and addition of transgenes to specific genomic loci. >> Category: Biotech Research
  • Genomic Approaches for Engineering of Metabolic Pathways of Medicinal Plants    By: Dr.Vinay Kumar

    This article focused on the functional genomic approaches for engineering of secondary metabolic pathways of medicinal and aromatic plants. Genetic engineering of a secondary metabolic pathway aims to either increase or decrease the quantity of a desired compound or group of compounds for production of novel compounds of medicinal significance. >> Category: Biotech Research
  • Transgenics: Concerns and Prospects    By: Dr. Shiv Kumar

    The article talks about concerns and prospects of transgenics in general. The focus of article is how to restore the confidence and trust of consumer by educating him and what potential benefits can be tapped using this powerful technology in evolving regulatory mechanism of developing countries. >> Category: Biotech Research
  • Molecular Markers and their Utilization in Plant Breeding    By: Dr. Vikas Gupta

    Molecular markers usage now a days in Plant breeding is a routine activity. A brief introduction about molecular markers and their utilization in plant breeding is discussed. >> Category: Biotech Research
  • Glycomics - An Overview of the Carbohydrate Study    By: Divya Narayan

    Glycomics not only involves the study of carbohydrates alone, but also deals with the study of carbohydrates combining or being associated with other macromolecules - lectins, glycoproteins, glycolipids, enzymes specific for carbohydrates, cell receptors specific for carbohydrates, sugar molecules present in nucleic acids, etc. >> Category: Biotech Research
  • Genome Engineering    By: Rhitu Rai

    This article focuses on new age techniques for editing the genome in vivo which require targeting DNA breaks to defined location followed by either Non homologous end joining or homologous recombination. The origin and basis of TALENs is discussed. >> Category: Biotech Research
  • Role of Beta-glucosidase in Preparing Lactose-Free Pasteurized Milk    By: Hareepriyaw M

    The enzyme lactase or Beta-galactosidase is released by the intestinal villi and it hydrolyzes the lactose into galactose and glucose. Lactase is known to be responsible for the digestion of bovine milk consisting of 4.8% of lactose, on average. Lactose fermentation and malabsorption of lactose in the gut results from the deficiency of the gene responsible for this protein. >> Category: Biotech Research
  • E3 Ubiquitin Ligase Increases Thermal Resistance in Plants    By: Hareepriyaw M

    The increase in global temperatures has shown negative influence on the agricultural production all over the world. Developing thermal resistance in crops has become a great challenge for plant biology researchers and in agriculture. >> Category: Biotech Research
  • Bio-engineering of Buoyant Gas Vesicle Nanoparticles From Haloarchaea    By: Hareepriyaw M

    Gas vesicles are organelles of bacteria and archaea that constitute protein nanoparticles. Gas vesicles are known to provide flotation to the microorganisms, so that oxygen and light are properly available to them especially in phototrophic organisms. Gas vesicles are useful in biotechnological applications as their protein membrane is stable against solubility and they are produced in large scale. The hypotonic lysis of the host can lead to the purified gas vesicles. >> Category: Biotech Research
  • Microbial Biosynthesis of Rare Hexoses    By: Hareepriyaw M

    Monosaccharides and their derivatives are referred to as rare sugars. Monosaccharides have applications in nutrition and pharmaceutical industries and they are used as precursors for drug candidates and natural products. The availability of these rare sugars is restricted and the enzymatic and microbial transformations have become vital tools to synthesize these rare sugars. >> Category: Biotech Research
  • Targeted Disruption of Gene in Bacillus Anthracis    By: Hareepriyaw M

    Bacillus anthracis is a gram+ve bacterium and exists mostly in spore form. The spores will come in contact with the wounds or food of the animals and will enter into the animal. The entered spores will further germinate to a vegetative organism. Most of the infections or 95% of the infections in animals occurs through cutaneous entry while rarely infections occur through pulmonary or gastrointestinal paths. >> Category: Biotech Research
  • Aluminium Resistance in Crop Plants    By: Hareepriyaw M

    Aluminium resistance is found to be governed by genes that belong to two different gene families which encode for transport proteins. Enough data is currently available for increasing aluminium resistance of crop species with the help of biotechnology. This study focuses on the progress made in the research related to aluminium resistance in plants, the genes that are controlling relevant processes and the genetic engineering approaches to increase aluminium resistance in crop plants. >> Category: Biotech Research
  • LecRK-1.9 Enhances Resistance of Solanaceous Plants Against Phytophthora Infestans    By: Hareepriyaw M

    The potato cultivars that are susceptible to Phytophthora infestans were the end products of the potato plants bred for resistance and monitored for the gene called race-specific late blight resistance or R. The R gene belongs to the gene class called nucleotide binding site-leucine rich repeat (NB-LRR). This gene was observed to originate from Mexican wild potato plants like Solanum stoloniferum and S.demissum. >> Category: Biotech Research
  • Production of L-Ornithine by Evolving Cornybacterium Glutamicum    By: Hareepriyaw M

    L-ornithine is an important component of the urea cycle and is a non-essential amino acid. In this study, speE gene was knocked out from the C.glutamicum ATCC 13032 strain deficient in argF and proB genes. The resultant mutant is made to grow in an adaptive environment to evolve metabolically and improve the production of L-ornithine in it. >> Category: Biotech Research
  • Transient Expression of Recombinant Antibodies in HEK-293 Cells    By: Hareepriyaw M

    Human Embryonic Kidney 293 cells or HEK 293 cells, are very easy to grow and transfect very readily and have been widely used in cell biology research for many years. >> Category: Biotech Research
  • Histamine Releasing Factor-Therapeutic Target For Asthma    By: Hareepriyaw M

    Translationally controlled tumor protein or TCTP is otherwise called as a histamine releasing factor (HRF). The basophils taken from allergic donors were releasing histamine induced by lavages of nose and bronchoalveoli, and skin blister fluids. These donors were called as HRF responders while those people having basophils not responding to histamine releasing factors are called as HRF non-responders. HRF is expressed everywhere and is an intracellular protein. >> Category: Biotech Research
  • Effective Transduction by Lentiviral Vectors    By: Hareepriyaw M

    Lentiviral vectors are generated by transfection of vectors with long terminal repeats along with a transgene in a vector construct called as transfer vector >> Category: Biotech Research
  • Estimation of Endo-1,4-beta-xylanase in Ustilago Maydis    By: Hareepriyaw M

    The microorganisms can convert biomass components like cellulose and hemicellulose into chemical substances. Ustilago maydis can grow on Xylan and release certain chemicals that are of biotechnologically beneficial. This study focuses on identifying the enzyme endo-1,4-beta-xylanase involved in degradation of Xylan. >> Category: Biotech Research
  • Genetic and Genomics Resources in Finger Millet    By: Dr. Amolkumar U. Solanke

    Finger millet is serving as a staple food for millions of poor people living on the most marginal agricultural lands. Owing to its ability to grow under varied agro-climatic and resource poor conditions, it can be a valuable resource for understanding genomics of tolerance to various abiotic stresses and important genes and alleles from finger millet can be utilized for crop improvement. >> Category: Biotech Research
  • Expression of Proapoptotic Protein hPNAS-4 in E. Coli    By: Hareepriyaw M

    The current study deals with the purification, characterization, subcellular localization and polyclonal antibody production of hPNAS-4 protein. The purified hPNAS-4 protein and the synthesis of polyclonal antibodies are powerful tools for studying the apoptotic mechanisms of hPNAS-4. >> Category: Biotech Research
  • Marker-Free Transgenic Plants    By: Dr. Suresh Kaushik

    Public concerns about the issue of the environmental safety of genetically modified plants have led to a demand for technologies allowing the production of transgenic plants without selectable (antibiotic resistance) markers. >> Category: Biotech Research
  • Random and Insertional Mutagenesis for Functional Genomics in Plants    By: Dr. S. V. Amitha Mithra

    The turn around time of structural genomics in plants and animals has been coming down in exponential manner since the advent of New Generation Sequencing technology. However, the functional genomics is not keeping pace with the advancement of structural genomics. Mutagenesis, both random and insertional, is one of the important means for functional genomics of all organisms. >> Category: Biotech Research
  • Efficient Purification of Alpha Amylase by Affinity Precipitation    By: Hareepriyaw M

    Alpha-amylase is one of the many industrial enzymes that is utilized in several industrial applications like high-fructose corn syrup, desizing of textiles, ethanol production, detergents, hydrolysis of oil field drilling fluids, recycling of paper and modified starches. >> Category: Biotech Research
  • Growth of E.coli in the medium with Tender coconut water    By: Hareepriyaw M

    Tender coconut water (TCW) with Nitrogen source an economical growth medium for the production of recombinant proteins in Escherichia coli. >> Category: Biotech Research
  • Genome Sequence of Cucumber Reveals Mechanism of Monoecy    By: Dr. Prasanta K. Dash

    Cucumber Genome has been decoded by a hybrid approach of genome sequencing. Subsequently, resequencing of 115 cultivars across the globe revealed the mechanism of evolution, absence of whole genome duplication, formation of special organs and mechanism of sexual dimorphism in this important crop of cucurbitaceae family. >> Category: Biotech Research
  • Impact of Phaseolus Vulgaris Extract on Cyanobacterial Growth    By: Hareepriyaw M

    Phaseolus vulgaris consists of phytohaemagglutinin or PHA. PHA is a lectin that has its role in cell proliferation and defence mechanisms. The present study concentrates on protein synthesis and growth of Nostoc ellipsosporum NCIM 2786 on the Fog’s medium which is supplemented with the extract of Phaseolus vulgaris rich in phytohaemagglutinin and glucose. Statistical analysis of medium components was performed by Plackett-Burman method. >> Category: Biotech Research
  • Engineering Bt Toxins for Effective Insect Control    By: Rhitu Rai

    This article focuses on biology and genetic engineering of the most successful biopesticide Bacillus thuringiensis and how biotechnological tools have helped in improving upon the Bt toxin's efficiency as well as widening the host spectrum. Few strategies to cope with resistance development in insects are also dealt with. >> Category: Biotech Research
  • Method of Effective DNA Isolation from Terminalia arjuna    By: Hareepriyaw M

    Terminalia arjuna is commonly called as Roxburgh and grows to a height of 20 to 30 meters. It belongs to the plant family of Combretaceae. The bark of Terminalia arjuna is useful in treating heart failure, coronary artery diseases, hypercholesterolemia and more >> Category: Biotech Research
  • Antimicrobial and Antioxidant Features of Nutmeg    By: Hareepriyaw M

    Nutmeg is the dried seed of the Myristica fragrans Houtt. It has anti-cancer, anti-inflammatory and anti-diarrheal properties. Nutmeg also acts as a memory enhancer and aphrodisiac. This study concentrates on investigating the antioxidant and antimicrobial properties of nutmeg with the help of extracts of methanol, ethanol, acetone, butanol and nutmeg aqueous extracts. >> Category: Biotech Research
  • Protein Bodies in Transgenic Plants | Case Study-Tobacco Plant    By: Hareepriyaw M

    Elastin like polypeptide and hydrophobin fusion proteins expressing transgenic tobacco plants consisted of intracellular protein bodies. >> Category: Biotech Research
  • Zinc Finger Nuclease (ZFN) - Modify human genome precisely and efficiently    By: Priyanka Chaudhary

    It is possible to target the DNA sequences probing gene function. Designer zinc finger proteins (ZFPs) are the sequence specific tools which are used to manipulate a gene of interest at genomic level. Several methods are being used to modify the human genome precisely and efficiently. These methods hold great promise to revolutionize the gene therapy arena. One of these promising technologies ZFN. >> Category: Biotech Research
  • Sponge Associated Microbes and its Biotechnological Potential    By: Balaram Mohapatra

    This article focuses on microbes those are symbiotically associated with marine sponges in deep oceans and their novel as well as biotechnological potential. As marine environment is most diverse and well maintained in terms of energy, productivity, homeostasis and eco sustainability, it is important to study the microbial diversity, their beneficial biotechnological potential for sustainability >> Category: Biotech Research
  • Epilepsy Gene LGI2    By: Shivani Sharma

    After the discovery of LGI2 (the gene linked to epilepsy), major principles can be postulated in the field of therapeutics. Not only can a better understanding can be ensured to be gained of epilepsy disorder and mechanisms but also improved medications and treatments can be developed in order to reach the target of curing the vast majority of people suffering from this very disease >> Category: Biotech Research
  • Common Feed Additives in Animal Nutrition    By: Bimrew Asmare

    A range of feed additives, many of biotechnological origin, are known to modify rumen fermentation. These biotechnological products can help in improving animal feed its nutrients e.g. silage, biological treatment with microorganisms or through modifying the digestive and metabolic systems of the animals, i.e. modifying rumen ecosystem, to enable them to make better use of the available feeds. Therefore, supplementation of the diet as a means of improving nutritive value is becoming commonplace. The use of feed additives to improve post ingestion quality of feeds is on the verge of delivering practical benefits to animal production systems. >> Category: Biotech Research
  • DNAzymes: Introduction and DNAzymes in Cancer Treatment    By: Priyanka Chaudhary

    DNAzymes are the highly useful tools in molecular biology and now-a-days they are being used in clinical research. DNAzymes are the potent analytic tool available to molecular biologists. There are several techniques which are used for gene silencing which include DNAzymes, short interfering RNAs (siRNAs), antisense oligonucleotides and the ribozymes mainly. >> Category: Biotech Research
  • Transfer of Male Sterility to Cauliflower Varieties Through Inter-Generic Crosses    By: Dr. Swati Saha

    Male sterility described as inability of the plant to produce fertile pollen is widespread in angiosperms and provides one of the most efficient means of directed pollination for the large-scale production of hybrid seeds in crops. Male sterility manifests itself usually in floral development as an incompatibility of nuclear-mitochondrial interaction in alloplasmic lines derived from wide hybridization, which carry nuclear and mitochondrial genomes from different species. >> Category: Biotech Research
  • Tomato Genome For Comparative Genomics    By: Dr. Amolkumar U. Solanke

    The tomato genome sequenced by International Tomato genome Sequencing Consortium presently serves as a powerful resource to identify gene functions and clone agronomically important genes and alleles not only from tomato but also from less studied crop species through comparative analysis across the Solanaceae species. >> Category: Biotech Research
  • SNPs in Complex Plant Genomes - Discovery and Genotyping    By: Dr. S. V. Amitha Mithra

    Next Generation Sequencing (NGS) technologies have made whole genome sequencing and SNP discovery in many crop species possible. Still there are a lot of issues to be resolved in complex genomes, which is more prevalent in angiosperms, regarding sequencing and SNP discovery . These issues and the way forward are discussed in this article. >> Category: Biotech Research
  • Omics Approaches for Identification of Stress Responsive Genes    By: Dr. Amolkumar U. Solanke

    Comparative study of transcripts, proteins and metabolites in stressed and un-stressed crop plants help to pick important stress associated genes, products which can be useful in providing various levels of tolerance. These three omics approaches have a fundamental difference from genomics in that these are dynamic in nature where as genome is static across the tissues and lifetime of an individual.. >> Category: Biotech Research
  • Green Fluorescent Protein (GFP) - The Protein That Revolutionized Science    By: Shivani Sharma

    Ever since the study of biochemical and molecular mechanisms inside living beings started, a need was felt to examine these processes more closely so as to understand the life better. From the propagation of a disease-causing pathogen/molecule inside the body to development of a particular type of cell. How a cell initiates its functions inside body, how the developmental stages occur in a cell, h >> Category: Biotech Research
  • Genetic Transformation Using Microinjection    By: Shivani Sharma

    Genetic transformation of animals is the introduction/removal of new genes into the genome of an organism. This is also widely referred to as genetic engineering. This genetic manipulation doesn't have any effects on the other genes' working and the rest of the genome functions in a usual manner. >> Category: Biotech Research
  • Biotechnological Approaches for Isolating the Genes of Agronomic Significance    By: Dr. N. C. Gupta

    Identification and isolation of the novel genes from different plant species is an important aspects of the molecular biology research. This article mainly emphasize the various biotechnological approaches those has been been routinely utilized for cloning the genes of agricultural importance. >> Category: Biotech Research
  • Characterization of a Plant RNA Pol-II Promoter Sequence    By: Dr. N. C. Gupta

    Detailed analysis of a promoter sequence is usually required to firmly validate their function as well as decipher the way for their possible implication in crop improvement program. >> Category: Biotech Research
  • Isolation of a Plant Promoter by T-DNA Insertional Mutagenesis    By: Dr. N. C. Gupta

    Promoter of a gene plays an important role in regulating the expression behavior of a gene and determines when, where and to what extent that gene will be expressed. There are several strategies known to identify a plant promoter in which insertional mutagenesis approach is comparatively technically less demanding and more expedient. Once the promoter trapped line becomes available one of the appr >> Category: Biotech Research
  • Epigenetics: Applications in Crop Improvement    By: Dr.Sunil S Gomashe

    The article deals with role of epigenetics in crop imrovement and its wide applications >> Category: Biotech Research
  • Agricultural Biotechnology: From Green Revolution to Gene Revolution    By: Dr. Suresh Kaushik

    The gains in food production provided by the Green Revolution have reached their ceiling while world population continuous to rise. Agricultural biotechnology is that area of biotechnology involving application to agriculture. New developments in agricultural biotechnology are being used to increase the productivity of crops .. >> Category: Biotech Research
  • Successful Single Cell Protein Production From Pineapple Waste    By: Shivani Sharma

    Single cell protein production is at the peak of infancy today. The production of single cell proteins can be accelerated by genetically modifying the micro-organisms employed for the purpose according to the need. >> Category: Biotech Research
  • Blue Eye Mutation: Inheritance From a Single Ancestor    By: Shivani Sharma

    The blue eye mutation dates back to 6-10 thousand years ago when the main gene associated with the eye color, OCA2, underwent a mutation in a single human. Ever since that single event, no mutations have arisen in this gene and thus is considered most likely to be the cause of all the blue-eyed humans alive presently. >> Category: Biotech Research
  • Salmonella - From a Disease to a Cure    By: Maitree Baral

    Salmonella has set milestone in the biotech research; starting from a disease causing agent to an anti cancer agent. >> Category: Biotech Research
  • Bioreactor Operations- Vortex Flow Separation For Separating Cells    By: Meenu Kumar

    The technique of culturing mammalian cells on a large-scale is frequently performed in airlift reactors or stirred tank in batches. In order to achieve product concentrations and high cell densities fed-batch processes were introduced. >> Category: Biotech Research

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