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  • Aptamers : A Novel DNA-RNA Approach in Biotechnological Research    By: Sandhya Anand

    Aptamers are oligonucleotide sequences carrying out specific functions. These novel molecules are selected from random pools of such DNA/RNA sequences based on their binding ability and are emerging fast as new method of therapy. >> Category: Biotech Research
  • Some Genetic Manipulations of Phosphate Solubilizing Bacteria    By: Sonali Bhawsar

    Investigation of mps genes from different PSB presents great potential for future improvement of PGPR strains as efficient bioinoculants for sustainable agriculture. In the present article, various attempts of genetic manipulation of PSB have been discussed. >> Category: Biotech Research
  • Microbial Phosphate Solubilization    By: Sonali Bhawsar

    Phosphorus is treated by PSM in 3 different ways: solubilization, mineralization and immobilization. Insoluble phosphates are dissolved by acidification, chelation or proton extrusion mechanisms. Solubilization by means of acidification of growth medium, that means the production of organic and inorganic acids is the most common mechanism found in PSM. >> Category: Biotech Research
  • Symbiotic Associations of Bacteria    By: Sonali Bhawsar

    It would be very interesting to know about symbiotic associations of bacteria with different animals including human beings, protozoa, algae, fungi and plants. You will see nature's very complex but highly coordinated interactions of different living forms. There could be more examples of bacterial associations with other organisms; only few of them are described in this article to get an idea about versatility of bacterial interactions. There could also be a possibility of existence of novel interactions which we still have not been discovered so this would be one of the areas of investigation for enthusiastic researchers. >> Category: Biotech Research
  • Bacterial Antifreeze Proteins and Its Applications    By: Shekhar C Bisht

    Antifreeze proteins (AFPs) are structurally diverse group of proteins that have the ability to modify ice crystal structure. AFPs create a difference between the melting point and freezing point known as thermal hysteresis (TH) is a measure of the antifreeze activity. >> Category: Biotech Research
  • Structural Feature of Cold Actives Enzymes    By: Shekhar C Bisht

    Enzymes are biological catalyst and involved in most of the chemical reaction in the cell, those are necessary for the cell survival. Adaptation of the cell to the low temperature calls for presence of intracellular enzymes, which are active at low temperature. A wide range of molecular determinants confer conformation flexibility of enzymes. These determinants involved in protein stability are ei >> Category: Biotech Research
  • Improving Micronutrients Acquisition Efficiency Through Transgenic Technology    By: Dr. Suresh Kaushik

    Advancements made in recent years in the area of recombinant DNA technology have provided an altogether new dimension to agricultural research. For instance, engineering plants with improved nutrient uptake may help in sustainable agriculture in the twenty-first century. Molecular genetic tools have given the researcher the possibility to identify key regulatory steps in the acquisition of nutrients by plants. There are now convincing evidences that the genes coding for several target traits, mainly the transporters and other mechanisms for nutrients acquisition opens up several options to improve nutrient uptake and utilization in soils with low fertility. The transfer of the corresponding genes to agriculturally important crops might therefore allow to increase their nutrient uptake capacity. >> Category: Biotech Research
  • The Process of Brewing: Technical Aspects and Market Potential    By: Sonali Bhawsar

    This article gives an elaborate account of the process of brewing. Making of beer and wine is described in detail. It also deals with information regarding byproducts, diseases and market value of alcohol production process. >> Category: Biotech Research
  • Proteomics - A Promising Approach of Biotechnology    By: Shikha Sharma

    The advent of large-scale DNA sequencing has transformed biological research in a short span of time. With the advancement in the discovery of most human genes, the next necessity is to explore the key molecules (proteins) responsible for biological problems so as to achieve an in-depth knowledge of complex biological processes. In this article, the crucial contribution of proteomics to our understanding of biology and medicine, through the global analysis of gene products, is discussed. >> Category: Biotech Research
  • Algal Biotechnology - Importance and Applications    By: Sonali Bhawsar

    Algae do not require prepared and fertilized land for their growth. They can grow on marginal land like salinated or drought affected hard soils. They also grow in waste water effluents or sewage or even waste water from nuclear reactors. >> Category: Biotech Research
  • Solid-Phase Oligonucleotide Synthesis    By: Shikha Sharma

    One of the experimental techniques that played a key part in the rapid development of genetic engineering is the chemical synthesis of oligonucleotides of defined structure. The chemistry of deoxyoligonucleotide synthesis has been the subject of study for many years. Currently, the phosphoramidite method of chemical DNA synthesis is the procedure of choice. Chemical synthesis takes place in the 3'-5' direction (reverse of the biological polymerization direction). >> Category: Biotech Research
  • Animal Pharming: Projects, Commercial Products and Ethical Issues    By: Akash Mukherjee

    This is the second article of a 2-part series. This series is on Animal Pharming, and the problems that arise while doing it. It also covers some application based examples of it, along with the bioethical issues associated with it. This section deals with some interesting pharming projects, commercial products, bioethical issues and the bottomline. >> Category: Biotech Research
  • Animal Pharming: Problems, Transgenic Animal Creation and Procedure    By: Akash Mukherjee

    This is the first article of a 2-part series. This series is on Animal Pharming, and the problems that arise while doing it. It also covers some application based examples of it, along with the bioethical issues associated with it. This section deals with introduction to pharming, problems with bacerial cultures and the procedure of transgenic animal creation. >> Category: Biotech Research
  • Shikimate Pathway in Vibrio Cholerae    By: Akash Mukherjee

    This is an article on the Theoretical-background of one of my unpublished research on structure determination of 3-Dehydroquinate dehydratase. Bioinformatical results can be made available on request. >> Category: Biotech Research
  • Biostatistics - Statistical Analysis in Life Processes    By: Zandro Cabaral

    This article is an outlook on what is biostatistics and how it is used in biotech researches. Biostatistics is the science which deals with the plans and methods of collecting, tabulating, and analyzing numerical facts and figures in life processes. >> Category: Biotech Research
  • Potency of Guava Extract Against Streptococcus Mutans (Tooth Decay)    By: Zandro Cabaral

    Article about the study of antimicrobial potency of guava extract to treat and fighting tooth decay due to Streptococcus Mutans >> Category: Biotech Research
  • Psychrophilic Enzymes - A Boon to Modern Industry    By: Pournami Gouthaman

    Psychrophilic enzymes are cold active biocatalysts obtained from cold loving microbes. These enzymes have extensive application in various processes in food and dairy industry, textile industry, laundry, etc. Because of its unique optimal growth temperature at cold conditions, it is widely used as alternative for normal mesophilic enzymes in many industrial processes. >> Category: Biotech Research
  • HER-2 a Predictive Marker For Breast Cancer Treatment    By: goldy yadav

    Cancer is a deadly disease in which disorder occurs in the normal process of cell division, which is controlled by the genetic material (DNA) of the cell (1). There are over 100 different types of cancer, and each is classified by the type of cell that is initially affected. Cancer harms the body when damaged cells divide uncontrollably to form lumps or masses of tissue called tumors. >> Category: Biotech Research
  • Herbicide Resistance for Weeds | Strategies for Glyphosate Resistance    By: Shikha Sharma

    Glyphosate is a glycine derivative and acts as a broad spectrum herbicide and is effective against 76 of the world's worst 78 weeds. Weeds are unwanted and useless plants that grow along with the crop plants. Weeds compete with the crops for light and nutrients besides harboring various pathogens >> Category: Biotech Research
  • Human Immunodeficiency Virus: HIV Discovery and Immunological Aspects    By: Shikha Sharma

    With the advent of 1980s, a new immunodeficiency syndrome (AIDS) has been recognized and the same has been found to affect many populations worldwide and killing many of them. Then hundreds of scientists around the world started looking for the cause of this dreaded disease. clear. >> Category: Biotech Research
  • Advancements in Biotechnology - At a Glance    By: Shikha Sharma

    Biotechnology as the name indicates is the assemblage of technology in science of biology. Modern Biotechnology initiated with the discovery of double helical structure of the DNA. Subsequent investigation that helped in unraveling the process of inheritance pattern provided impetus to biotechnological research. >> Category: Biotech Research
  • Ribozymes: Molecular Scissors For Genetic Manipulations    By: Shikha Sharma

    Ribozymes are the RNA molecules that like enzymes (protein) are capable of catalyzing a biological reaction and are also called molecular scissors. Ribozymes catalyze a particular reaction either by the hydrolysis of one of their own phosphodiester bonds or by hydrolysis of bonds in other RNA. >> Category: Biotech Research
  • Insect Resistance - Current Perspectives in Research    By: Shikha Sharma

    It is estimated that about 15% of the world crops yield is lost due to insects. The damage is mainly caused by insect larvae and to some extent by adult insects. Initially, only the chemical pesticides are used to control the insect pests. But there are various drawbacks associated with its use. So keeping all these facts in view, scientists are working in search of new biotech alternatives. >> Category: Biotech Research
  • Reporter Genes - Indicators of Transformation    By: Shikha Sharma

    A reporter or marker gene is a gene, which produces a specific phenotype, in turn enables the differentiation of the cells possessing this particular gene from those without this gene. Hence, the transformed cells can be selected easily among the thousands of non-transformed cells. Reporter genes are an invaluable tool to track and study another associated gene in cell culture, animals and plants. >> Category: Biotech Research
  • Enzyme Immobilization - Methods and Applications    By: Shikha Sharma

    Enzyme immobilization is one of the most promising approaches for exploiting enzyme-based processes in biotransformation, diagnostics, pharmaceutical and food industries. Several hundred of enzymes have been immobilized in a variety of forms including penicillin G acylase, lipases, proteases, invertase, etc. and are being currently used as catalysts in various large scale processes. >> Category: Biotech Research
  • Antisense Technology - A Breakthrough Research    By: Shikha Sharma

    Antisense technology offers almost unlimited scope for the development of new methods of drug design. It is one of the most approved approaches among several others, for inactivating a single chosen gene. It has wide applications in crop improvement, treatment of genetic disorders, viral resistance, gene regulation etc. >> Category: Biotech Research
  • Difference Between Scanning Tunneling and Atomic Force Microscopes    By: Muniba Safdar

    Information about Scanning Tunneling Microscopy (STM) and Atomic Force Microscopy (AFM) ;their advantages & disadvantages, resolution, composition and applications. >> Category: Biotech Research
  • Comparison Between Scanning Electron and Transmission Electron Microscopes    By: Muniba Safdar

    Information about Transmission Electron Microscope (TEM) and Scanning Electron Microscope (SEM); their differences, crystallographic information, composition and applications. >> Category: Biotech Research
  • Biotechnology Impact on Alzheimer's Disease    By: Amna Adnan

    Alzheimer disease is the disease of brain or in other words it is neurodegenerative disorder. Biotech has played an important role in the treatment of this disease. >> Category: Biotech Research
  • Medicinal Plants: Source Of Medicine    By: goldy yadav

    Medicinal plants are always has been the exemplary source of medicine. Medicinal plants have pharmacological activities of possible therapeutic use. Careful and planned investigations of the plants are needed in order to develop new drugs that meet the criteria of modern treatment. >> Category: Biotech Research
  • Mtt Assay: Assess The Viability Of Cell In Culture    By: goldy yadav

    For assaying a compound toxicity (e.g anticancer compound) in in-vitro cell culture experiment, generally we used MTT assay it is commonly know as cell viability,and proliferation assay MTT assay is the most common reaction in the cell culture procedure. MTT stands for (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide). >> Category: Biotech Research
  • Breast Cancer: Cactus For Womens Life    By: goldy yadav

    Breast cancer is a malignant proliferation of epithelial cells lining the ducts or lobules of the breast. There are different types of breast cancer, with different stages (spread) I and II, aggressiveness, and genetic makeup.Worldwide, breast cancer is the second most common type of cancer after lung cancer (10.4% of all cancer incidence, both sexes counted) and the fifth most common cause of cancer death. The responsible factor for the breast cancer is the HER receptor family. HER family receptors are located on the surface of the cell. >> Category: Biotech Research
  • Gene Transfer Techniques: Biolistics, Bacterial and Viral Transformation    By: Hannibal Musarurwa

    Genetic engineering is largely depended on the gene transfer techniques. This paper gives a brief description on the most used methods in biotechnology laboratories. The techniques vary from biolistics, bacterial and viral transformation. >> Category: Biotech Research
  • Stem Cells From Bone Marrow and Vein Leftovers Can Heal Damaged Hearts    By: Amna Adnan

    Stem cells are the center of attention these days as they are providing opportunity of repairing the damaged heart tissues. Scientists are now able to use stem cells for the cure of heart disease. >> Category: Biotech Research
  • Extraction of Nucleic Acids (DNA and RNA) From Plant Tissues    By: Lorato Lekgari

    After plant transformation, a researcher has to ascertain that the gene has been cloned into the plant's genomic DNA and that it is being expressed. To do these, DNA and RNA has to be isolated and analysed. The CTAB extraction method is discussed. >> Category: Biotech Research
  • Apoptosis and Cancer: A Review    By: Robby Kumar

    The programmed cell death, also known as Apoptosis is a complex mechanism by which cell undergo its own destruction to control the process of cell proliferation or it can be due to in response of DNA damage. Apoptosis also plays an Important role in tissue homoeostasis and development. The most striking feature of the cancer cells is that they do not undergo Apoptosis. >> Category: Biotech Research
  • Gene Isolation and Characterisation    By: Lorato Lekgari

    For a genetic engineer to transform organism, a gene of interest has to be isolated isolated and characterised,then its suitability for the purpose of the manipulation has to be verified. Here we briefly look at how gene of interest can be isolated and characterised >> Category: Biotech Research
  • Plasmid as Vectors For Plant Transformation    By: Lorato Lekgari

    Plasmids are found in microorganism mainly in bacterial cells. They are naked double stranded DNA molecules that replicate independently of their host's DNA but cannot live outside the host. >> Category: Biotech Research
  • PCR Aspects and its Future | PCR versus Cloning    By: Muniba Safdar

    PCR is the abbreviation of 'Polymerase Chain Reaction'. It was invented by Kary Mullis in 1983. It is one of the methods of genetic engineering used to produce multiple copies of DNA, other being the cloning. It is used to amplify specific nucleic acids. >> Category: Biotech Research
  • DNA Sequencing: Maxam Gilbert Method    By: Farah Mahboob

    When DNA's double helical structure was discovered by Watson and Crick, scientists entered a race to sequence the human genome. Among the first techniques developed for DNA sequencing was the Maxam Gilbert method developed in 1973. >> Category: Biotech Research
  • Biological Activities and Bioassays    By: Lorato Lekgari

    Discusses bioassays used to determine biological activities of plant extracts, especially important in medicinal plants. Anti-inflammatory and Anti-oxidant activity. >> Category: Biotech Research
  • Treatment of Heart Disease With Stem Cells    By: Amna Adnan

    stem cells are making it possible to treat different life threatening diseases like cancer and heart disease. Bone marrow stem cells are taken from the patient and are injected directly into the heart. It is a very successful method of heart surgery. >> Category: Biotech Research
  • Polymerase Chain Reaction (or PCR) - Technique For Amplifying DNA    By: VIVEK VYAS

    Polymerase chain reaction or PCR in biotechnology is a technique of Amplifying single piece of Deoxyribose nucleic acid (DNA), into million copies of DNA. Well known applications of these techniques are DNA cloning and sequencing, gene functional analysis, hereditary diseases diagnosis, genetic finger printing (forensic studies) .... >> Category: Biotech Research
  • Tissue Cells and Skin Cells Reprogrammed Into Embryonic Stem Cells:-    By: Amna Adnan

    Stem cells are the undifferentiated cells which differentiate into different types of cells when the embryo develops. Researchers have successfully reprogrammed the tissue cells and skin cells into embryonic stem cells. >> Category: Biotech Research
  • Biotechnology as a Tool in Medicine: Focus on Artemisinin    By: Lorato Lekgari

    Artemisinin is a secondary metabolite produced by Artemisia annua, and it was used in Chinese ancient medical remedies. Biotechnology techniques are now being used to improve the production of this highly important and significant compound in the medical/pharmacological sector. It is used as treatment against malaria which is a diseases that kills a lot of people yearly. >> Category: Biotech Research
  • Nanoparticles as Delivery Device For Gene Therapy    By: Amna Adnan

    Nanoparticles are very useful as they provide safe way of gene therapy. They ar non viral vectors and have no side effects unlike viruses which retain their infection ability. Scientists have combined calcium phosphate and lipid shell to create nanoparticle and inserted desired DNA in it. Without any harmful effects, it reaches to its target and replaces the damaged DNA. >> Category: Biotech Research
  • Human Longevity: A Revolution in Biotechnology and Nanotechnology.    By: VIVEK VYAS

    Life span varies from one animal to another . The key to this can be genes responsible for the same . Biotechnology and nanotechnology application can help in longevity ( Life span) increase with use of these genes . >> Category: Biotech Research

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