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City: New Delhi |
State: New Delhi
About Me: Scientist at National
Research Centre on Plant
New Delhi. My area of work
is to Increase the crop
productivity by utilizing
India is one of the leading producers of oil seed Brassicas accounting for 11.12 % of the world's rapeseed-mustard production, and ranks third in the world next to China and Canada and Sclerotinia stem rot disease is a menace during late flowering and maturity of plants resulting up to 80% loss of yield causing a major drop in oil seed economy.
Identification and isolation of the novel genes from different plant species is an important aspects of the molecular biology research. This article mainly emphasize the various biotechnological approaches those has been been routinely utilized for cloning the genes of agricultural importance.
Detailed analysis of a promoter sequence is usually required to firmly validate their function as well as decipher the way for their possible implication in crop improvement program.
Promoter of a gene plays an important role in regulating the expression behavior of a gene and determines when, where and to what extent that gene will be expressed. There are several strategies known to identify a plant promoter in which insertional mutagenesis approach is comparatively technically less demanding and more expedient. Once the promoter trapped line becomes available one of the appr