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Author: Dr. Suresh Kaushik

Author Photo Total Articles: 23 | Total Views: 83636 | Points Scored: 95,136

City: New Delhi | State: Delhi

About Me: A researcher and teacher with Ph.D. in Molecular Biology and Biotechnology from New Delhi.

Articles by Dr. Suresh Kaushik:
  • New Plant Breeding Techniques to produce New Improved Crops ( 726 reads)   

    Many new plant breeding techniques such as cisgenesis, intragensis and genome editing are currently applied as potential alternative to transgenesis to generate engineered crops with various new traits. Crops produced using some these techniques cannot be distinguished from their conventionally bred counterpart and therefore there are claims that they should be exempted from the GMO legislation - Category: Biotech Research
  • Opening Black Box of Soil Microbial Diversity through Molecular Techniques ( 647 reads)   

    The vast majority of microbes have not yet been isolated, identified or characterized. The advent of culture-independent techniques has transformed the field of microbiology and soil microbial ecology in particular. PCR-based techniques such as DGGE, TGGE, T-RFLP, allow the classification of microorganisms based on particular genetic markers such as 16S rRNA and the profiling of complex microbial communities on the basis of sequence diversity. - Category: Biotech Research
  • Biotechnology for Enhancing Micronutrients Acquisition and Uptake Efficiency ( 526 reads)   

    Improvement of micronutrient acquisition in area where micronutrients deficiency in soils limits crop productivity is probably the most challenging and rewarding areas of research to achieve the sustainable productivity of agricultural crops. Progress made in recombinant DNA technology in recent years and the application of molecular techniques has advanced our understanding in unraveling the mechanisms of acquisition of micronutrients by the plants from less-labile of soil pools and role of genes involved in these processes, and have provided an altogether new dimension to agricultural research. - Category: Applications
  • Intelligent Nano-Fertilizers ( 2744 reads)   

    The plant needs different amount of nitrogen depending on its growth stage. Nitrogen-use efficiency for most crops ranges from 30 to 50 percent. A new generation of fertilizers will increase this efficiency from 30 percent to upwards of 80 percent. Smart biosensors and smart delivery systems will help in enhancing productivity in agriculture. Intelligent nano-fertilizers can reduce the amount of nitrogen lost during the crop production. - Category: Nanotechnology
  • Arsenic Speciation Analysis using High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry ( 1378 reads)   

    Speciation is the analytical activity of identifying and/or measuring in a sample the quantity of one or more individual chemical species. Arsenic has different toxicological properties dependent upon both its oxidation state for inorganic compounds as well as the different toxicity levels exhibited for organic arsenic compounds. HPLC is the technique of choice in modern speciation analyses due to their resolution and the ease with which they are coupled to ICP-MS, allowing for on-line separation and detection. - Category: Toxicology
  • Arsenic Poisoning - Effects on Human Health ( 1307 reads)   

    This article describes the essential background and toxicity of arsenic in the environment. Arsenic has different toxicological properties dependent upon both its oxidation state for inorganic compounds as well as the different toxicity levels exhibited for organic arsenic compounds. Some currently used chromatographic and mass spectrometry applications as well as sample handling procedure necessary to detect and quantify arsenic in its various chemical forms - Category: Healthcare
  • DNA Metabarcoding: A Rapid Method of Biodiversity Assessment ( 2250 reads)   

    DNA metabarcoding refers to the automated identification of multiple species from a single bulk sample containing entire organisms. This offers unprecedented scientific and operation opportunities in order to understand biodiversity distribution and dynamics in a better way. - Category: Biotech Research
  • Nanotechnology: Applications in Agriculture ( 6740 reads)   

    Nanotechnology has the potential to revolutionize the agriculture with new tools for the rapid disease detection and their treatments, enhancing the ability of plants to absorb nutrients, increasing the efficiency of pesticides and herbicide. The advancement in agricultural nanotechnology to promote 'precision farming' allowing optimum use of the natural resources with judicious farming practices; and different sensor and controlled delivery technologies could change the face of farming so-called 'Nanobio-farming' - Category: Nanotechnology
  • Detection of Genetically Modified Foods ( 4876 reads)   

    The detection and identification of GMOs in foods and other materials are derived by legal requirement and marketing demands. In order to test the amount GMOs in a consistent and reproducible way, suitable analytical methods are required. Foods derived from GM crops or raw materials such as grains are identified by tests for the presence of introduced DNA or by detecting expressed novels proteins. - Category: Biotechnology products
  • Marker-Free Transgenic Plants ( 3064 reads)   

    Public concerns about the issue of the environmental safety of genetically modified plants have led to a demand for technologies allowing the production of transgenic plants without selectable (antibiotic resistance) markers. - Category: Biotech Research
  • Engineering Crops for Efficient NPK Capture From Soils ( 2489 reads)   

    Creating designer crops with enhanced nutrient uptake could help in reducing the need for fertilizer application. Key regulatory steps in the acquisition of nutrients by plants have been identified. The genes coding for several target traits, mainly the transporters, opens up several options to improve nutrient uptake and utilization in soils with low fertility. The transfer of the corresponding genes to agriculturally important crops might allow to increase their nutrient uptake capacity. - Category: Agriculture
  • Nitrate Pollution in Ground Water ( 3547 reads)   

    Nitrate ion, the end product of oxidation process of nitrogen, is a plant nutrient, but beyond certain limits in the ground water, it is a potential threat to human health. Nitrate itself is harmless but when it is reduced to nitrite, it causes a rare disease in infants known as Methaemoglobenama (Blue Baby Syndrome) and stomach cancer in adults.It is perceived mainly as a chemical fertilizers used by farmers, but much of nitrate found in soil is produced by the microorganisms that decompose plant residues and other nitrogen containing residues present in the soil. Nitrate pollution in ground water is rising at an alarming rate in India. - Category: Biology
  • Gene Cloning in Plasmid Vector ( 4695 reads)   

    The terms recombinant DNA technology, DNA cloning, molecular cloning, or gene cloning all refer to the same process: the transfer of a DNA fragment of interest from one organism to a self-replicating genetic element such as a bacterial plasmid (cloning vector). The DNA of interest can then be propagated in a foreign host cell. This technology has been around since the 1970s. Gene cloning has contributed hugely to agricultural and medical research. It has mainly been applied in agriculture to develop resistance to pests and diseases and improve nutritional quality of crops. It has allowed genes and their functions to be studied in much greater detail than was previously possible. - Category: DNA
  • Metagenomics: New Challenges Ahead in Molecular Soil Ecology ( 2973 reads)   

    Metagenomics is the culture-independent analysis of a mixture of microbial genomes using an approach based either on expression or on sequencing. This involves isolating DNA from an environmental sample, cloning the DNA into a suitable vector, transforming the clones into a host bacterium and screening the resulting transformants. Two types of screening have been used to identify clones carrying desired traits from metagenomics libraries: sequenced based analysis and functional based analysis. It is the daunting task of understanding the genomics of uncultivated microorganisms or whole environmental genomes with respect to identifying the functions of genes as compared with a well-studied and easily cultivated microorganism. Metagemomics produces a snapshot of the microbial community genome at a specific point in time and space. - Category: Environmental Biotechnology
  • Agricultural Biotechnology: From Green Revolution to Gene Revolution ( 3141 reads)   

    The gains in food production provided by the Green Revolution have reached their ceiling while world population continuous to rise. Agricultural biotechnology is that area of biotechnology involving application to agriculture. New developments in agricultural biotechnology are being used to increase the productivity of crops .. - Category: Biotech Research
  • Inductively Coupled Plasma - Mass Spectrometry ( 2670 reads)   

    Inductively coupled plasma mass spectrometry (ICP-MS) is a type of mass spectrometry which is capable of detecting metals and several non-metals at concentrations as low as one part in 1012 (part per trillion). This is achieved by ionizing the sample with inductively coupled plasma and then using a mass spectrometer to separate and quantify those ions. It is undoubtedly the fastest growing trace element technique available today. It allows determination of elements with atomic mass ranges 7 to 250 and its ability to carry out rapid multi-elements determination at the ultra-trace level have made it very popular in diverse range of applications areas including environment, geochemical, semiconductor, metallurgical, nuclear, chemical, climatic and biotechnology. - Category: Environmental Biotechnology
  • Genomics Tools For Microbial Ecology: New Directions ( 2452 reads)   

    Microorganisms have been integral to the history and function of life on Earth. They have played important roles in Earth's climate, geological, geochemical and biological evolution. Microbial ecology examines the diversity and activity of microorganism in Earth's biosphere. The applications of genomics tools such as DNA cloning, DNA sequencing, polymerase chain reaction, DNA re-association kinetics analysis, fluorescent in situ hybridization (FISH), microarray technology, representational difference analysis, 2-D gel electrophoresis, mass spectrometry and gas chromatography have revolutionized microbial ecological studies. - Category: Biotech Research
  • 16S RRNA Gene: Application in Soil Microbiology ( 3487 reads)   

    Soil is one of the most diverse habitats on Earth and understanding the biodiversity of soil is difficult because of the heterogeneity that exists on both local or geological scales. The 16S rRNA is a ribonucleic acid molecule found in the ribosome. The 16S rRNA ribosomal RNA gene sequences of bacteria and achaea have been the primary tools for identifying the populations inhabitating soil and for monitoring microbial community dynamics. Therefore, 16S rRNA gene is the preferred gene target for identification of bacterial species, defining microbial community diversity and establishing phylogenetic relationship between unknown and uncultivated soil microorganisms. - Category: DNA
  • RNA Editing - Mechanisms, Features and Significance ( 6448 reads)   

    The information flows directly from DNA to protein through the RNA intermediate molecule. But, it has been discovered that the information that is contained in the DNA is not always found in the RNA products used to make proteins. Mitochondria and chloroplast contain the biochemical machinery to alter the sequence of the final transcription product. This process is called RNA editing. The primary benefit of RNA editing could be evolutionary conservation of protein structure. - Category: DNA
  • Seed Storage Proteins ( 5739 reads)   

    A major part of the human diet all over the world consists of cereals and legumes. Seed storage proteins are important for human nutrition but generally incomplete in nutrition due to their deficiency in several essential amino acids, for example, lysine and tryptophan in cereals and methionine and cysteine in legumes. Modern biotechnology offers alternative approaches for rectifying this nutrition deficiency. - Category: Biotechnology products
  • Recombinant Proteins Produced in Genetically Engineered Crops ( 2474 reads)   

    Production of recombinant proteins in seed for genetically enhanced plants will revolutionize the industry with biotech products that are easier to produce, safer and cost less to produce. Use of plants as a recombinant protein expression system will hold great promise for the future. - Category: Biotech Research
  • Improving Micronutrients Acquisition Efficiency Through Transgenic Technology ( 5389 reads)   

    Advancements made in recent years in the area of recombinant DNA technology have provided an altogether new dimension to agricultural research. For instance, engineering plants with improved nutrient uptake may help in sustainable agriculture in the twenty-first century. Molecular genetic tools have given the researcher the possibility to identify key regulatory steps in the acquisition of nutrients by plants. There are now convincing evidences that the genes coding for several target traits, mainly the transporters and other mechanisms for nutrients acquisition opens up several options to improve nutrient uptake and utilization in soils with low fertility. The transfer of the corresponding genes to agriculturally important crops might therefore allow to increase their nutrient uptake capacity. - Category: Biotech Research
  • Phytoremediation - Use of green plants to remove pollutants ( 13874 reads)   

    The major challenge facing society in the twenty-first centaury is to feed and provide for increasing numbers of people while protecting human health and the environment. Over the last centaury, increasingly wide spread heavy metal pollution and organic contaminants have caused many areas to become non-arable and hazardous for both wildlife and human populations. Industrial waste products account for most heavy metals pollution, although sewage and household waste also introduce substantial levels of toxic metals into the environment. Contaminated soils and waters pose a major environmental human problem. Physical, chemical and biological processes are already being used to remediate contaminated soil, but these techniques can grossly disrupt the polluted site and are expensive. This problem may be solved by the emerging phytoremediation technology that employs the use of higher plants for the cleanup of contaminated environments. - Category: Environmental Biotechnology

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