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City: Muzaffarpur |
About Me: Senior Scientist (Plant
Pathology) at ICAR-National
Research Centre on Litchi,
working on diseases of
litchi (Litchi chinensis),
and mycorrhizal association
in litchi. Earlier, worked
on management of aflatoxins
Trichoderma is a free-living cosmopolitan fungal species interacting with roots, soil and foliar environments. They grow rapidly and have good rhizosphere competence with other microorganisms. They inhibit or degrade pectinases and other enzymes that are essential for phytopathogenic fungi. Trichoderma has gained maximum attention as biocontrol agent because of its effectiveness against large number of soil-borne phytopathogenic fungi, suppressive effects on some root nematodes without adversely affecting beneficial microbes like Rhizobium, activating host defense system and capable of promoting growth of certain crops.
With the availability of monoclonal and polyclonal antibodies against aflatoxins, various simple, sensitive and specific enzyme linked immunosorbent assays (ELISAs) have been developed. This is rapid and relatively inexpensive assay method and results are also acceptable. This article provides a simplified account of the protocol for assay of aflatoxins in peanut by indirect competitive ELISA.
Some of the laboratory and field techniques with respect to aflatoxin research in peanut are described. The article will be very useful to students, teachers and researchers particularly beginners.
This article provides a simplified account on aflatoxins, their biosynthetic pathway and mechanism of action. The article is intended for beginners such as students, teachers and researchers.
A study was undertaken in India to determine the effectiveness of sorting and grading procedures prior to blanching, followed by a combination of manual and electronic eye sorting (camera sorter) after blanching to get rid of the aflatoxin contaminated kernels from the bulk peanut lot. The study conclusively proved that the blanching used in conjunction with manual and electronic sorting was very effective in eliminating aflatoxin-contaminated kernels.