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Author: Nidhi Uppangala

No Photo Total Articles: 129 | Total Views: 1686872 | Points Scored: 1,751,372

City: India | State: India

About Me: I'm a BIOTECHNOLOGY Post Graduate.

Articles by Nidhi Uppangala:
  • Animal Cell Culture Media : Natural and Artificial Media ( 85332 reads)   

    Animal cell culture can be described as in vitro maintenance and propagation of animal cells using a suitable nutrient medium. Culturing is a process of growing animal cells artificially. The most important and essential step in animal cell culture is selecting appropriate growth medium for invitro cultivation. - Category: Others
  • Hydrophobic Interaction Chromatography (HIC) - A Protein Separation Technique ( 8364 reads)   

    Hydrophobic interaction chromatography is used to separate proteins with difference in hydrophobicity. This technique is ideally used as intermediate step in a purification process or to capture specific protein. The separation is based on the reversible interaction between the protein and the hydrophobic surface of a chromatographic medium. - Category: Others
  • Structural Basis of Proteins ( 4220 reads)   

    Proteins are biomolecules present in all living cells and play a very important role in its functioning and also its survival. Proteins are made up of linear chains of amino acid molecules, which are linked together by a special bond known as polypeptide bonds. - Category: Biology
  • Plasma Membrane and Integral Membrane Proteins ( 7699 reads)   

    Cells are the basic unit of life. All cells have a common feature known as outer selective permeable membrane called as cell membrane or plasma membrane. Almost all eukaryotic cells contain more complex and complicated system of internal membranes. These internal membranes give rise to membrane covered various compartments within each cell. Cell membranes are mainly composed of lipids and proteins - Category: Biology
  • Artificial Retina, Limb and Body Part Development ( 6907 reads)   

    The development of artificial organs in the laboratory is the main invention in the medical science. An artificial organ is a man-made organ that can be used to replace the torn or damaged natural organ in humans. - Category: Biotechnology products
  • Purifying Technique of Biomolecule Protein ( 6521 reads)   

    To know about the working mechanism of a protein, it is very much essential to know about its amino acid sequence and also their nature. Once the purification of a protein is completed, then it is very easy to determine the amino acid sequence. Proteins are purified using appropriate purifying techniques. - Category: Others
  • Biosensors - Immunosensors, Biochip and Bioreporter ( 5093 reads)   

    A gene based biosensor is used to monitor and optimize bioremediation of some of the complex chemicals such as polycyclic aromatic hydrocarbons (PAHs), benzene, toluene and also carcinogens. A university researcher is testing the effectiveness of genetically engineered Pseudomonas fluorescens in degrading the contaminant polycyclic aromatic hydrocarbons. - Category: Others
  • Artificial Organ Development - Blood Vessels, Bones and Skin ( 8161 reads)   

    One of the important discoveries of new age medical science is the development of artificial organs in laboratory. These artificial organs can be implanted in human body to replace the natural organ. New research is being conducted to improve functions of these artificial organs. In the near future we would be able to create a real human being using these artificial organs. - Category: Biotechnology products
  • Single Cell Protein- Advantages and Disadvantages ( 57650 reads)   

    The need for large scale production of single cell protein is the need for more protein in the diet of humans. Single cell protein typically refers to proteins extracted from pure culture or mixed culture of microorganisms such as algae, yeasts, fungi or bacteria. These extracted protein are used as a substitute for protein-rich food in humans and as well as animal feeds. - Category: Biotechnology products
  • Uses of Lipases in Biotechnology ( 8956 reads)   

    Enzyme lipases play a major and important role as biocatalysts in biotechnology applications. Lipases are used in biotechnology applications such as synthesis of biopolymers, production of biodiesel, the production of pure pharmaceutical compound, agrochemicals and also flavour compound. - Category: Biotechnology products
  • Artificial Womb, Heart and Blood Development ( 5127 reads)   

    Artificial organ development in laboratory conditions is one of the most fascinating branches in medical science. Artificial organs are man-made organ in vitro conditions and are implanted in human body to replace the natural ones. - Category: Biotechnology products
  • Characteristics and Applications of Biosensors ( 16851 reads)   

    Technically speaking biosensor is a probe that integrates biological components with an electronic component so that we can get a measurable signal. Biological components may include a whole bacterial cell or biological products such as antibody or an enzyme. - Category: Others
  • Applications of Bioinformatics in Biotechnology ( 28581 reads)   

    Bioinformatics has got so many applications in biotechnology field. Some of the important ones include automatic genome sequencing, automatic identification of genes, identification of functions of gens, predicting the 3D structure modelling and pair-wise compairism of genes. - Category: Bioinformatics
  • Issues and Benefits of Human Cloning Technique ( 8817 reads)   

    Human cloning can be defined as creating a genetically identical copy of a human or a cell or tissue. There are many types of human cloning such as therapeutic cloning, reproductive cloning and replacement cloning. - Category: Others
  • Production of Organic Acid and Biopolymer ( 7125 reads)   

    Microorganisms are used in the production of organic acid and also biopolymers. These products have changed our life. Microorganisms are used in industrial microbiology techniques to produce important pharmaceutical compounds, food additives, biofuels, agricultural products and much more. - Category: Biotechnology products
  • Somatic Cell Nuclear Transfer ( 5119 reads)   

    Somatic cell nuclear transfer is a technique used to produce cloned cells. In this procedure that is in somatic cell nuclear transfer, the nucleus from a somatic cell is transferred into an enucleated oocyte. This transfer helps in reprogramming of an enucleated oocyte into an embryonic cell. - Category: Others
  • Antibiotics and Amino Acid Production Using Microorganisms ( 14909 reads)   

    Microorganism is used to produce products such as antibiotics and amino acids in large quantity. These products have changed our lives and also increased our life span. Microorganisms are also used to produce products such as food additives, biofuels, industrial products, agricultural products and also pharmaceutical products for human as well as animal health. - Category: Biotechnology products
  • Difference Between Prokaryotic and Eukaryotic mRNA ( 100657 reads)   

    How Prokaryotic and Eukaryotic Messenger Ribonucleic Acid differ? Messenger RNA is a single stranded structure, with no base pairing. It contains bases such as adenine, guanine, cytosine and uracil. Since mRNA is transcribed from the DNA molecule, its sequences are complementary to that of DNA on which they are transcribed. - Category: Genetics
  • Ion Exchange Chromatography: A Biotechnology Technique ( 7925 reads)   

    Ion exchange chromatography is used for separating proteins with different charge and this gives a very high resolution separation with high sample loading capacity. The separation of proteins is based on the reversible interactions between a charged chromatographic medium and with an oppositely charged protein molecule. - Category: Others
  • Biotechnology and Environment Protection ( 12664 reads)   

    Environment has been contaminated with non biodegradable compounds due to the revolution in science and technology. Most common contaminates found in the environment are commercial hydrocarbons, Organo-halogenated compounds like trichloroethylene, tetrachloroethane and much more. All these environmental contaminants can be degraded by using a biotechnology technique known as bioremediation. - Category: Environmental Biotechnology
  • Maintenance of Seed Stock and Large Scale Culture of Cell Lines ( 5711 reads)   

    A number of cell lines both finite and continuous are developed from human tissues as well as other animal tissues. Continuous cell lines are developed originally from tumor tissues. Cell lines are characterized before preserving or distributing them for further research work. A cell line can multiply and divide to form seed stock and also working (distribution) stock. - Category: Others
  • Mode of Action of Antibiotics ( 34542 reads)   

    Antibiotics are chemical molecules or compounds that specifically targets and kill cells. Antibacterial action generally follows some of the mechanisms such as inhibition or regulation of enzymes involved in the synthesis of cell wall, nucleic acid synthesis and repair, or protein biosynthesis. Antibiotics target the cell functioning of rapidly dividing cells. - Category: Biology
  • Analysis of Genes and Gene Transcripts ( 3899 reads)   

    In biotechnology applications and many experiments require the use of cloned DNA. Therefore analyzing required gene of interest and also its transcripts are very important to the experiments. Some of the methods used to analyze the gene and its transcripts are described in this article. - Category: Genetics
  • Production of Tissue Plasminogen Activator and Interferon ( 19914 reads)   

    Most important application of biotechnology technique called as recombinant DNA technology is the production of most important biomolecules such as insulin, follicle stimulating hormones, blood products like clotting factor VIII, albumins, tissue plasminogen activators and also cytokines and also interferons, colony stimulating factors and much more. - Category: Biotechnology products
  • Recombinant Clotting Factor VIII, Erythropoietin and Hepatitis B Vaccine ( 13282 reads)   

    Biotechnology technique known as recombinant DNA technology are used in the large scale production of important biomolecules that can be used to improve the quality of life of humans. - Category: Biotechnology products
  • Three Phase Protein Purification Strategies ( 8786 reads)   

    The three phase purification strategy has been used in simple laboratory purification to large or industrial scale production. This three phase purification system is also used to purify large scale production of pharmaceutical compounds or biomolecules such as monoclonal antibodies or any recombinant enzymes. - Category: Others
  • Identifying a Specific Clone in CDNA and Genomic Library ( 10562 reads)   

    A number of techniques have been developed by scientists for identifying the specific gene of interest in cDNA as well as genomic library. In this article some of the techniques are discussed - Category: DNA
  • Plasmid and Bacteriophage λ as Cloning Vectors ( 24822 reads)   

    Many types or kinds of cloning vectors are used for the transfer of gene of interest into the desired tissue or cell. Some of the examples for vectors used in recombinant DNA technology are plasmids, phagemids, cosmids, shuttle vectors and much more. - Category: Others
  • Applications of Molecular Imaging Technique ( 3105 reads)   

    Molecular imaging technology can be used for imaging gene delivery, exogenous marker gene and also to understand the molecular pathways like angiogenesis, apoptosis and much more. - Category: Healthcare
  • Physiological and Chemical Properties of Animal Cell Culture Media ( 18838 reads)   

    The ideal animal cell culture medium is expected to possess certain physiological and chemical properties such as pH, oxygen, carbon dioxide, buffering, osmolality, viscosity, temperature in such as way that it should support growth and proliferation of cultured animal cells. - Category: Others
  • Hybridoma Technology - A Biotechnology Technique ( 45189 reads)   

    Hybridoma technology is used to produce a hybrid cell. These hybrid cells are produced by fusing B-lymphocyte with tumour cell and they are called as myeloma cells. Thus these hybrid cells have got the ability to produce antibodies due to the B-lymphocyte genetic material and also capacity to divide indefinitely in the culture due to the presence of tumour cell - Category: Others
  • Immunosuppressant Sirolimus Production and Its Uses ( 5884 reads)   

    Immunosuppressant Sirolimus is also known as rapamycin, discovered as a product of Streptomyces hygroscopicus bacterium obtained from the soil. Streptomyces hygroscopicus belong to of actinomycetes. - Category: Biotechnology products
  • Clonal Growth of Animal Cells in Semisolid Culture Media ( 5142 reads)   

    Animal cell lines like HeLa cells and L-cells, and also normal cells which are transformed by viruses can grow and proliferate to form colonies in soft agar-based media. Most of the normal cells cannot grow in this media except some type of cells like cartilage cells. Isolation of clones is very easy, if they are cultured in semisolid media. - Category: Others
  • Issues Related to Attachment-dependent Animal Cell Culture ( 6586 reads)   

    Animal cell culture can be done by two methods, namely attachment-dependant animal cell culture and suspension animal cell culture. Both the ways has got some issues. But current article talks about the issues related to the attachment-dependent animal cell culture method. - Category: Issues
  • Recombinant Protein Expression System ( 18598 reads)   

    Recombinant proteins are the ones which are produced by genetically modified organisms, after inserting the appropriate gene into their genome.Recombinant proteins are expressed or produced in large quantities using expression systems such as E.coli, yeast, filamentous fungi, mammalian cells, insect cells and also plant cells are used. - Category: Biotechnology products
  • Somatic Cell Fusion- A Biotechnology Technique ( 29639 reads)   

    Somatic cell fusion or Hybrid cells can be produced by fusing different types of somatic cells from two different tissues or species in a cell culture media. Usually human somatic cells like fibrocyte or leucocyte are fused with continuous cell lines of mouse. - Category: Biotechnology products
  • Cultivating Stem Cells in the Laboratory ( 4871 reads)   

    Stem cells are unspecialized cells capable of dividing and renewing themselves for a number of cell cycle. When stem cells replicate many times, it is called as proliferation. A starting population of stem cells after many months of proliferation in the laboratory or in vitro condition can yield millions of cells. - Category: Stem Cells
  • Production of Recombinant Human Growth Hormone Somatotropin ( 41302 reads)   

    Recombinant human growth hormone is generally produced by inserting the human growth hormone gene into plasmids of E.Coli bacteria. Recombinant bacterial cells are cultured and human growth hormones produced by these bacteria are extracted from the extracellular media. - Category: Biotechnology products
  • Applications of Animal Cell Culture Technique ( 62117 reads)   

    Animal cells extracted from their tissue or organs are cultured in aseptic laboratory with environmental conditions same as in vivo and this technique is called as animal cell culture. Some of the important areas where cell culture plays an important role are toxicity testing, cancer research, virology, gene therapy, drug discovery and many more. - Category: Others
  • Issues Related to Suspension Animal Cell Culture ( 7346 reads)   

    Animal cell culture is a technique used to culture animal cells outside the tissue or organ from which they are extracted. Animal cell are cultured mainly by two methods, such as suspension culture and anchorage dependant culture. But both type of culture method have got some issues. This article talks about the issues related to suspension culture method. - Category: Issues
  • Cryopreservation and Conservation of Plant Genetic Material ( 13714 reads)   

    Cryopreservation is a technique used to preserve cell or tissue using liquid nitrogen. Temperature is maintained as low as 77K, at this temperature all the biological activity of a cell or tissue is stopped. - Category: Agriculture
  • Cancer Stem Cells and Cancer ( 3939 reads)   

    Cancer stem cells are the one from which tumors originate, these tumor cells have same properties like normal stem cell such as multipotency and self-renewal ability. Scientists are arguing that cancer is a stem cell disorder and not a simple disruption in the cell mechanism - Category: Stem Cells
  • Stem Cell Research and Its Therapeutic Applications ( 4910 reads)   

    Stem cells are undifferentiated cells with the ability to divide many times by mitosis. When applied sufficient amount of growth factors along with cell growth inducing factors, these stem cells will differentiate into specialized cells, such as heart muscle, cartilage, nervous system, pancreas or skin cells. - Category: Stem Cells
  • Stem Cell Culture in Laboratory ( 8433 reads)   

    Growing cells in the laboratory environment, by providing appropriate nutrients, temperature, growth factors and some other chemical substances is called as cell culture. Not only differentiated animal cells, but also stem cells can be cultured in laboratory in aseptic condition. - Category: Stem Cells
  • Enzymes in Diagnosing Disease ( 33566 reads)   

    Enzymes are biocatalysts and are made up of amino acid or they are proteins. All enzymes are protein but all proteins are not enzymes. Enzymes can be differentiated between one another and they can be used as diagnostic tool to detect some diseases in human. - Category: Healthcare
  • An Introduction to Drug Discovery Technique ( 3392 reads)   

    Drug discovery is a process of discovering or designing new drugs or pharmaceutical molecules. In new approach or now a day's new drugs are discovered by understanding how disease or infection can be controlled at the molecular and physiological level and then target are identified based on this knowledge. - Category: Healthcare
  • Genetically Modified Crops as Medicine ( 13903 reads)   

    Genetically modified plants or transgenic plants contain a gene or genes from another species. T Transgenic plants are produced in such a way that they produce antibodies, enzymes, and this also helps in plant research. - Category: Agriculture
  • Recombinant Interferon as Drugs ( 7961 reads)   

    Interferons are produced in large quantities using recombinant DNA technology and they are used as drugs to treat various types of diseases. Many of these recombinant interferons are given subcuteniously or intramascularly to treat diseases. - Category: Healthcare
  • Enzymes Used in DNA Manipulation ( 22700 reads)   

    Enzymes are used in molecular biotechnology or genetic engineering to manipulate biological material DNA. Enzymes like nucleases, ligases, polymerases, Modifying enzymes and topoisomerases are commonly used enzymes to do the job such as DNA manipulation. - Category: Genetics
  • Genetic Testing in Detecting Genetic Disorder ( 4067 reads)   

    Genetic testing is done to see if an individual is at risk for certain types of diseases like cancer by looking for certain gene mutations. Genetic testing is also used to confirm gene mutation present in an individual or in a family. - Category: Genetics


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