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Most Recent Biotech Research Articles.
  • Gene Switching and GURTs: What, How, and Why?    By: Deepak Vishwanathrao Pawar

    Gene switching in plants describes a wide range of mechanisms to control gene expression for purposes beneficial to human beings and the environment >> Category: Biotech Research
  • CRISPR-Cas Technology: A Novel Approach for Plant Genome Modification [PDF]    By: Vyomesh Shailesh Patel

    CRISPR/Cas technology has made a breakthrough in the field of genome editing which has added new stars in transgenic technology. This article mainly deals with basic concept of CRISPR/Cas technology and its mechanism along with various types of CRISPR/Cas system. >> Category: Biotech Research
  • In-silico Gene Prediction Tools    By: Deepak Vishwanathrao Pawar

    Techniques of genome-wide in silico search for genes have greatly facilitated gene identification by analyzing genome sequences. >> Category: Biotech Research
  • Microbial Fermentation for Plant Nutrition and Battling Weeds and Pests    By: Deepak Vishwanathrao Pawar

    Microorganisms are often labeled as dangerous, they can be crucial in saving crops, increasing yields, and protecting soils. >> Category: Biotech Research
  • How Genetic Engineering is different from Conventional Breeding?    By: Deepak Vishwanathrao Pawar

    How genetic engineering different from conventional breeding? >> Category: Biotech Research
  • Nutrient Media for Plant Tissue Cultures    By: Dipti Raghunath Dhumale

    The article is depicting the use of different medium for plant tissue culture. >> Category: Biotech Research
  • High Throughput Phenotyping of Transgenic Plants    By: Ekatpure Sachin Chandrakant

    Plant phenotyping is an emerging science for the prediction of organism's phenotype using genetic information collected from genotyping. It's an important tool for non-destructive measurement of plants growth, morphology and physiology at large scale. >> Category: Biotech Research
  • Genetically Modified Mustard: Dhara Mustard Hybrid-11    By: Ravi Kumar

    DMH (Dhara Mustard Hybrid)-11 is genetically modified variety of mustard developed by Centre for Genetic Manipulation of Crop Plants at Delhi University under the supervision of Dr. Deepak Kumar Pental (Professor, Department of Genetics). It is developed by using three genes viz., barnase, barstar and bar from Bacillus amyloliquefaciens for hybrid seed production >> Category: Biotech Research
  • Molecular Breeding and Marker-Assisted Selection | Applications and Limitations    By: Deepak Vishwanathrao Pawar

    Phenotype may not always be a good indicator of genotype. The phenotypic evaluation of many traits is cumbersome, tedious and time-consuming. Selection for the desirable allele of a gene/quantitative trait locus (QTL) on the basis of molecular marker(s) linked to it in the place of phenotype generated by this allele is known as marker-assisted selection (MAS). >> Category: Biotech Research
  • Synthetic Chromosome: A tool for Crop Improvement    By: Utpal Roy

    Synthetic chromosome is a second generation transgenic approach which directly transfers large gene complexes or multiple genes by telomere mediated truncation. >> Category: Biotech Research
  • NGS and Bioinformatics Tools to Detect Mixed Virus Infections in Plants    By: Nabarun Roy

    Mixed infections by viruses are now-a-days a burning issue of research for the plant pathologists. For example, streak disease of tomato and Maize lethal necrosis (MLN) disease of maize are such diseases caused by mixed infections of 2 separate viruses. Mixed infection results in a synergistic increase of disease severity and ultimately kills the infected plant. Using of next generation sequencing (NGS) platforms and bioinformatics tools such as BLAST and MEGA can help in the early detection of mixed viral infections >> Category: Biotech Research
  • Diagnosis of Plant Diseases    By: Deepak Vishwanathrao Pawar

    If plant diseases are diagnosed early and correctly, treatments can be developed against these pathogens and could minimize losses. >> Category: Biotech Research
  • Virus-Based Vectors and their Application    By: Pooja Kumari

    Virus-based expression vectors are commonplace tools for the production of proteins or the induction of RNA silencing in herbaceous plants. It considers a completely different set of uses for viral vectors in perennial fruit and nut crops, which can be productive for periods of upto 100 years. Value of virus-based vectors is that they add nothing permanently to the environment. Perennial plants as a tool to assist in genetic improvement, for breedable crops, Vectors can be used to speed up sexual crosses. Furthermore, with continually emerging pathogens and pests, viral vectors could express genes to protect the plants or even to treat plants after they become infected. >> Category: Biotech Research
  • Reverse Genetics: From Genotype to Phenotype    By: Helen Baby Thomas

    The development of whole genome sequencing has enabled many researchers to have access to all of the gene sequences within a given organism and their function. So, instead of going from phenotype to sequence as in forward genetics, reverse genetics works in the opposite direction - a gene sequence is known, but its exact function is uncertain. In reverse genetics, a specific gene or gene product is disrupted or modified and then the phenotype is measured. >> Category: Biotech Research
  • Basic DNA Testing Process and its Importance    By: Cornelius Onye Nichodemus

    DNA testing process involves some set techniques required for the study of a DNA (genetic material of an individual). This process plays great role in fields of forensic science, agriculture, medicine etc for the genetic composition of individuals makes them uniquely different. >> Category: Biotech Research
  • Extraction Methods for Preparation of Bioactive Plant Extracts    By: Deepak Harishchandra Waghmare

    Extraction is the separation of medicinally active portions of plant using selective solvents through standard procedures. Various methods, such as Maceration, Infusion, Percolation, Decoction, Digestion, Soxhlet extraction, Microwave assisted extraction, Ultrasound-assisted extraction, Accelerated solvent extraction and Supercritical fluid extraction are used for the plant samples extraction which are discussed in this article. >> Category: Biotech Research
  • Journey of Immunoglobulin Protein: An Antibody to Plantibody    By: Dr. Kishor Uttamrao Tribhuvan

    Antibodies are group of glycoprotein produced by B-lymphocyte of vertebrate and it is the primary protection from pathogen infections. Ability of antibody to bind specific substrate increase its use as probe in research and diagnostics. Nowadays, production of antibodies is largely depend upon experimental animal which limit the quantity and also create ethical concern. Alternatively, transgenic plants having antibody producing genes can effectively synthesize heavy and light chain polypeptides and assembled into mature antibody called plantibody. >> Category: Biotech Research
  • Marker Assisted Selection and its Applications in Vegetable Crops    By: Pradeep Kumar Jatav

    It became possible to select desirable traits indirectly based on the banding pattern of marker instead of phenotype even at seedling stage which save time and labour cost, is termed as marker assisted selection. Easily detectable DNA markers can now be used in plant breeding >> Category: Biotech Research
  • Bio-PCR: Detection of Phytopathogenic Bacteria    By: Raj Kiran

    Bio-PCR is culturing of bacteria cells to the detectable limit prior to PCR, in turn increases sensitivity and allow early detection of bacterial pathogens. >> Category: Biotech Research
  • Bacterial Plasmid DNA and Conjugation Gene Transfer    By: Ashwini Kumar

    A plasmid is a molecule of DNA, independent of the bacterial nucleoid chromosome, which often has "bonus DNA" with instructions for weapons of infection. Both the bacterial nucleoid and bacterial plasmids are circular molecules of DNA - genetic information. Plasmids are, however, much smaller than the bacterial chromosome, usually only containing a few genes. Unlike the bacterial chromosome, plasmid traits are not normally required for the cell to function, but do help a bacterium survive and cause disease. Not all bacteria have plasmids, but those that do can share these advantageous molecules with other bacteria in a process called conjugation. >> Category: Biotech Research
  • Metabolomics for Rice Blast Resistance    By: Deepak Vishwanathrao Pawar

    Rice blast is one of the most devastating and destructive diseases of rice worldwide. Metabolomics can help to study Rice-M. oryzae interaction and thereby identify important candidates for resistance >> Category: Biotech Research
  • A Proteomic Road to Acquire Improvement in Crop Productivity [PDF]    By: Vinay Sharma

    The contribution of proteomic studies to our understanding of stress response mechanisms in different plant organs and tissues. The resilience of crop plants in the context of climate changes is absolutely essential to reach significant achievements in genomics-driven breeding of major crops for high productivity and stress tolerance. Moreover, dealing with complex and dynamic plant proteomes, it is crucial to choose suitable proteomic approaches targeting the identification of proteins and their modification that may contribute to crop improvement. >> Category: Biotech Research
  • Biogenesis of siRNA: Exogenous or Endogenous?    By: Dr. Kishor Uttamrao Tribhuvan

    siRNA generated in plants and animals not only exogenously originated in response to infection of viruses, bacteria or other parasitic organism but also it originated from genes located in pericentromeric, transposable element and repetitive region of the host genome. RNA polymerase IV and RNA polymerase V is responsible for biosynthesis of these endogenously originating siRNAs and processed in to mature and functional siRNA with the help of enzyme DCL3, AGO4 or AGO6 and RDR2. >> Category: Biotech Research
  • Genomic Innovation in Crop Improvement    By: Lalbahadur Singh

    Crop production needs to increase to secure future food supplies, while reducing its impact on ecosystems. Detailed characterization of plant genomes and genetic diversity is crucial for meeting these challenges. Advances in genome sequencing and assembly are being used to access the large and complex genomes of crops and their wild relatives. These have helped to identify a wide spectrum of genetic variation and permitted the association of genetic diversity with diverse agronomic phenotypes. >> Category: Biotech Research
  • Gel Electrophoresis Technique    By: Cornelius Onye Nichodemus

    Gel electrophoresis is a technique for separation and analysis of macromolecules and their fragments based on their size and charge. This technique is best carried out either through sharpie gel electrophoresis or polyacrylamide gel electrophoresis. >> Category: Biotech Research
  • Biotechnology Management: Current issues and its significance impacts on humans    By: Mercy Adusei Boatemaa

    The scope of biotechnology is a highly divergent technology and there must be an effective management of these scientific and engineering productions. Biotechnology management is the systematic way of planning, organizing, controlling and administering the application of scientific and engineering principles to process the materials by biological agents to provide goods and service. >> Category: Biotech Research
  • Phytohormones Regulation During Abiotic and Biotic Stresses    By: Era Vaidya

    Phytohormones regulate biotic and abiotic stress responses, independently and also via an efficient and coordinated genetic network. The different phytohormonal regulatory cascades are interconnected so as to activate stress responsive genes, in turn generating a physiological stress response. >> Category: Biotech Research
  • Transgenic Animals: A Short Review    By: Komal Ramchandra Pawar

    Animals those contain foreign gene are known as the transgenic animals. Until now there are different transgenic animals produced like mice, pig, cattle, fish, sheep, etc. These animals are produced for the study purpose, for the trait enhancement, or for the transgenic protein production. >> Category: Biotech Research
  • Micro-RNAs Controls Plant Development    By: Lalbahadur Singh

    In the long evolutionary history, plant has evolved elaborate regulatory network to control functional gene expression for surviving and thriving, such as transcription factor-regulated transcriptional programming. miRNAs controlled plant development, from miRNA biogenesis dysregulation caused pleiotropic developmental defects to specific developmental processes, such as SAM regulation, leaf and root system regulation, and plant floral transition >> Category: Biotech Research
  • New Plant Breeding Techniques to produce New Improved Crops    By: Dr. Suresh Kaushik

    Many new plant breeding techniques such as cisgenesis, intragensis and genome editing are currently applied as potential alternative to transgenesis to generate engineered crops with various new traits. Crops produced using some these techniques cannot be distinguished from their conventionally bred counterpart and therefore there are claims that they should be exempted from the GMO legislation >> Category: Biotech Research
  • Allele Mining: Strategies and Prospectus    By: Dr. Nalin K. Pagi

    Searching for different alleles. Allele - alternative forms of a genetic character found at a given locus on a chromosome. Mining - Searching. It is often referred to as 'dissection of naturally occurring variation at candidate genes/loci' or simply 'allele mining' Allele mining can be visualized as a vital link between effective utilization of genetic and genomic resources in genomics-driven modern plant breeding. >> Category: Biotech Research
  • Strategy to Produce Marker-free Transgenic Plants    By: Lalbahadur Singh

    Selectable marker genes (SMGs) have been extraordinarily useful in enabling plant transformation because of the low efficiency of transgene integration. However, there are perceived risks in wide-scale deployment of SMG-transgenic plants, and therefore research has recently been performed to develop marker-free systems. >> Category: Biotech Research
  • Origins of New Genes and Pseudogenes Increases Genome Complexity of Organism    By: Lalbahadur Singh

    The formation of new genes is a primary driving force of evolution in all organisms. How exactly do these new genes crop up in an organism's genome and what must occur in order for them to be passed on? >> Category: Biotech Research
  • Promoters Used in Gene Regulation and its Types    By: Ekatpure Sachin Chandrakant

    For the process of gene regulation there is need of regulatory elements such as promoters and cis-acting elements. Co-ordinate effect of these elements can induce the expression of gene. There are different types of promoters are available in molecular biology having its different functions and role in gene regulation. >> Category: Biotech Research
  • Gut Bacteria as Living Therapeuticals    By: Rajni Singh

    Certain genetic disorders like phenylketonuria and urea cycle disorder, sufficiently hamper the patient's normal life style, inspite of rigorous medication is still incurable. Serious cases leads to liver transplant and even in death. With the advancement of medical microbiology, the importance of gut microbes is being realised. These microbes are being genetically programmed to provide greater precision, safety and efficacy for disease treatment. Gut microbiota are useful in digestion, of certain food which cannot be digested normally and plays an important role in immune system >> Category: Biotech Research
  • TALENs: A Reliable Genome Editing Tool    By: Rhitu Rai

    The transformation from randome mutagenesis approach to efficient and specific gene targeting has been enabled by use of engineered nucleases. The article talks about its mechanism, principle and application. Further, TALENs classifies high for reliability due to its site specificity and low off target effects. >> Category: Biotech Research
  • Opening Black Box of Soil Microbial Diversity through Molecular Techniques    By: Dr. Suresh Kaushik

    The vast majority of microbes have not yet been isolated, identified or characterized. The advent of culture-independent techniques has transformed the field of microbiology and soil microbial ecology in particular. PCR-based techniques such as DGGE, TGGE, T-RFLP, allow the classification of microorganisms based on particular genetic markers such as 16S rRNA and the profiling of complex microbial communities on the basis of sequence diversity. >> Category: Biotech Research
  • Biosensors in Agriculture [PDF]    By: Deepali Rahi

    The agriculture industry has been for a long time dependent upon human expertise for quality control. Biosensors are analytical device which are designed for the measurement of various component of agricultural samples. Based on the principle of converting biological signal into electronic signal, different types of biosensors holds its applications in agriculture. >> Category: Biotech Research
  • Marine Algal Biotechnology and its Applications    By: Deepali Rahi

    Marine algae are untapped reservoir found in deep oceans can play a major role in primary as well as secondary sources in terms of food and bioactive compounds or biodiesel etc. respectively. Due to its wide range of adaptability in various environment it provides numerous possibilities. Biotechnological tools can help in cultivation and isolation of these bioactive compounds >> Category: Biotech Research
  • Virus Induced Flowering: Efficient Method of Transient Flower Induction    By: Ekatpure Sachin Chandrakant

    Naturally crop plants takes more time for flowering, that time cannot be reduced by conventional breeding practices for that reasons virus induced flowering (VIF) method could be used to accelerate flowering in plants and increase the progress of research and breeding >> Category: Biotech Research
  • Mysterious Applications of Cellular Mechanisms in Human Social Life    By: Swapnil Waghmare

    Micrometer sized cell can do many functions correctly. Cell has developed these mechanisms billion years ago. We can study these mechanisms to utilize in our social life. These mechanisms can be used to develop our defense system, communication system, energy production, transport etc >> Category: Biotech Research
  • PCR Based Markers: A great tool for Gene Tagging    By: Deepak Harishchandra Waghmare

    PCR based markers offers a quick and less expensive method for gene tagging. These markers are very much useful in molecular breeding for gene tagging and molecular assisted selection. >> Category: Biotech Research
  • Agroinfiltration - Effective Method of Gene Transfer    By: Komal Ramchandra Pawar

    Agroinfiltration is a method of gene transfer into plant. It is a very fast and effective method of gene transfer. There are two methods of agroinfiltration i.e. syringe agroinfiltration and vacuum agroinfiltration. >> Category: Biotech Research
  • Plant microRNAs: Sentinels in Plant Innate Immunity    By: Era Vaidya

    MicroRNAs regulate expression of a number of genes and have also been found to play a role in plant immune responses. A number of microRNA regulated immunity pathways operate in plants. These microRNAs modify gene expression on pathogen infection so as to help develop disease resistance in infected plants. >> Category: Biotech Research
  • CRISPER-Cas 9: A Possible Way for Precise Genome Editing    By: Bhoyar Pratik Istari

    The production of future genotypes is the way to sustain the present day agriculture. As the conventional breeding tactics not enough for the reason, the precise genome editing comes to the business which able to surpass the regulation. >> Category: Biotech Research
  • RNA Interference an Important Technology    By: Komal Ramchandra Pawar

    RNA interference is a potential technology for the production of desire phenotype. It is a tool used in biotechnology for the specific gene silencing. RNAi has broad applications for treating diseases and transgenic plant production. >> Category: Biotech Research
  • Genetics from Scratch to CRISPR    By: Fauzia Bi

    Science fiction became our reality and we don't even think about it. Genetic engineering have turned science fiction of genome editing [CRISPR], having a designer baby, in vitro fertilization, mass insulin production through bacteria ,GMO , tissue culture technique, and Synthia, [ the world's first synthetic life form ] , mutation breeding and transgenic organisms a reality. >> Category: Biotech Research
  • Metabolic Engineering of C3 Plants to Improve Crop Yield    By: Dr. Bunty Sharma

    Application of new technologies together with novel modeling approaches, increased yield through improved photosynthetic carbon fixation should be an attainable goal in the near to midterm. Engineering of C3 cycle offers an opportunity to increase photosynthesis and yield, which plays a central role in plant metabolism, providing intermediates not only for starch and sucrose biosynthesis, but also for isoprenoid metabolism and shikimic acid biosynthesis >> Category: Biotech Research
  • Polymer Production by Bacterial Strains    By: Rajni Singh

    As of late there has been a consistent quest for new water-solvent polysaccharides, especially those created by microorganisms. These biopolymers have the great interest due to their application potential in food, cosmetic, pharmaceutical and oil businesses, where they are utilized as thickening, balancing out and emulsifying operators oil industries. These biopolymers have the rheological properties so they are used for the identification of new production sources. >> Category: Biotech Research
  • Different Methods for Identification of Bacterial Plant Diseases    By: Dipti Raghunath Dhumale

    The article is informative and figures about different methods for detection of plant diseases. >> Category: Biotech Research

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