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Author: Waghmare Sandesh Tulshiram

Author Photo Total Articles: 5 | Total Views: 2004 | Points Scored: 4,504

City: Nashik | State: Maharashtra, India

About Me: I am currently working as Assistant professor at K.K. Wagh College of Agricultural Biotechnology, Nashik

Articles by Waghmare Sandesh Tulshiram:
  • Agrobacterium Mediated Gene Transfer: An Overview [PDF] ( 316 reads)   

    The bacterial genes are able to replicate along with the plant genome and uses the machinery of plants to express their genes in terms of the synthesis of a special class of compounds, called opines, which the bacterium uses as nutrients for its growth but are useless to the host cells. A. tumefaciens attracted to the wound site via chemotaxis, in response to a phenolic compound. The transfer DNA (T-DNA) is the transferred DNA of the tumour inducing plasmid (pTi) of some Agrobacterium species of bacteria. T-DNA has both its side 24 kb direct repeat border sequence and contains the gene for tumor / hairy root induction and also for opines biosynthesis. Removal of T-DNA region and other modification makes Ti plasmid as efficient gene cloning vector. - Category: Microbiology
  • Genetic Engineering Approaches For Tolerance Against Abiotic Stress ( 338 reads)   

    Abiotic stress is the negative impact of non-living factors on living organism in a specific environment. It is naturally occurring and often intangible factors.Abiotic stresses are the main factors that limit crop productivity. Drought, salinity and heavy metals stresses caused yield losses annually to a greater extent. Transgenic development is another straight forward technology to improve crop yield in abiotic stress affected land. The development of tolerant crops by genetic engineering, on the other hand, requires the identification of key genetic determinants underlying stress tolerance in plants and introducing these genes into crops. The advent of plant transformation may have placed within the grasp of the possibility of engineering greater abiotic stress tolerance in plants. - Category: Microbiology
  • Engineering Disease Resistance Based on TAL Effectors ( 293 reads)   

    TAL effectors affect plant processes in unique ways and the interaction between pathogen and host is now well understood in several specific cases. It provides a basis for overcoming TAL effector based virulence, exploiting TAL effector triggered resistance, and using TAL effector fusion proteins to engineer genomes for the specific purpose of aborting or limiting pathogen infection. TAL effectors have been shown to be a useful tool for genome engineering and synthetic biology in multiple systems - Category: Microbiology
  • Forward and Reverse Genetics Approaches in Functional Genomics ( 555 reads)   

    Functional genomics is characterized by high-throughput or large-scale experimental methodologies combined with statistical or computational analysis of the results. Functional genomics as a means of assessing phenotype differs from more classical approaches primarily with respect to the scale and automation of biological investigations. Forward genetics starts with identification of interesting mutant phenotype. Forward genetics seeks to find the genetic basis of a phenotype or trait. Automated DNA sequencing generates large volumes of genomic sequence data relatively rapidly. Reverse genetics starts with a known gene and alters its function by transgenic technology. Reverse genetics however, due to the advent of advances in sequencing is employed to seek out what sequences underlies the phenotype - Category: Genetics
  • RNA Editing and its Potential Role in Evolution ( 502 reads)   

    RNA editing is the post-transcriptional alteration of RNA sequences through insertion, deletion or substitution of nucleotides, excluding the RNA processing events. The gene whose transcript is edited is cryptogene and the addition of several Uracils during the insertion is termed as pan editing. Substitution includes both Cytosine (C) to Uracil (U) and Adenosine (A) to Inosine (I), through hydrolytic deamination. RNA editing could be used for genome manipulation in crop plants also. - Category: Microbiology

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