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Author: amar nagesh

No Photo Total Articles: 7 | Total Views: 2237

City: guntur | State: andhra pradesh

About Me: simple human being, i am in teaching profession, loves teaching. currently working in Biochemistry department at SSR Medical College, Mauritius, involved in various research activities, expert in writing review articles, visit me at www.am

Articles by amar nagesh:
  • Isolation of Plasmid DNA From E.Coli Part-2   

    Alkaline lyses method was used for the isolation of plasmid from bacterial cells. The method used was as described in cloning manual (Sambrook et al., 1989)... - Category: Biotech Research
  • Isolation of Plasmid DNA From E.Coli Part-1   

    Step by step procedure for the isolation of plasmid DNA from Escherichia coli. A plasmid is a small circular piece of DNA (about 2,000 to 10,000 base pairs) that contains important genetic information for the growth of bacteria. In nature, this information is often a gene that encodes a protein that will make the bacteria resistant to an antibiotic.A plasmid is a small circular piece of DNA (about 2,000 to 10,000 base pairs) that contains important genetic information for the growth of bacteria. In nature, this information is often a gene that encodes a protein that will make the bacteria resistant to an antibiotic. - Category: Biotech Research
  • Polymerase Chain Reaction - Primer Designing, Components and Steps of PCR   

    This article describes about steps of PCR, what is polymerase chain reaction, components of PCR, conditions required for PCR, and general PRIMER designing is explained. - Category: Biotech Research
  • Varieties of PCR and Their Primer Design - A Through Study | Part-2   

    This section discusses various PCR types including Hot Start PCR, Inverse PCR, Nested PCR and Reverse Transcription Polymerase Chain Reaction and its applications. - Category: Biotech Research
  • Varieties of PCR and Their Primer Design - A Through Study | Part-1   

    There are so many types of PCR avilable. In this article all the types of PCR and the designing of primers for each type of PCR has been described. This section discusses Allele-Specific PCR, Assembly PCR, Asymmetric PCR, Late-PCR and Colony PCR. - Category: Biotech Research
  • Central Dogma of Molecular Biology - What is Known Till Now?   

    The central dogma of molecular biology deals with the detailed residue-by-residue transfer of sequential information. Flow of biological information, Replication, Transcription, Translation are briefly described in this article - Category: Biotech Research
  • Principles of PCR - Polymerase Chain Reaction   

    Each and every laboratory will have different protocols for PCR operartion. In this article utility, principles and designing of primers in PCR is described - Category: Biotech Research



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